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对黄孢原毛平革菌(BKMF-1767)利用香蕉皮和玉米棒为发酵底物产胞外漆酶进行了研究.结果表明,当香蕉皮与玉米棒混合比例为1∶2、诱导剂CuSO4为0.4 mmol/L时,能获得最高漆酶酶活12.68 U/g.利用固态发酵所获得的粗漆酶液,进行了降解五氯酚的试验.在没有氧化还原介体时粗漆酶液能降解PCP,粗酶液中加入5 mmol/L氧化还原介体(ABTS)能获得更高的降解率,反应6 h分别为37.8%和97%.将粗漆酶液用(NH4)2SO4盐析纯化,用提纯后漆酶降解PCP,6 h后降解率为81.8%.
The results showed that when the mixing ratio of banana peel and corn cob was 1: 2, CuSO4 as the inducer 0.4 mmol / L, the maximum activity of laccase was 12.68 U / g.The crude laccase solution obtained by solid state fermentation was used to test pentachlorophenol degradation.Under the redox mediator, the crude laccase activity Degradation of PCP, crude enzyme solution by adding 5 mmol / L redox mediator (ABTS) to obtain a higher degradation rate, reaction 6 h were 37.8% and 97% of the crude laccase enzyme solution (NH4) 2SO4 salting-out After purification, the purified laccase degraded PCP, and the degradation rate was 81.8% after 6 h.