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应用马来丝虫成虫及牛丝虫成虫冰冻切片作荧光抗体试验(IFAT)及免疫酶染色试验(IEST),以检测对丝虫病人及健康人的敏感性、特异性和交叉反应性。两种抗原IFAT的敏感性和特异性分别为84.9～97.1％和90.3～97.1％;对钩虫、蛔虫感染者均无交叉反应。两种抗原IEST的敏感性和特异性分别为94.3～94.9％和97.1～100％;用牛丝虫成虫冰冻切片抗原更具有实用价值:IEST适用于现场。
In this study, the adult female Flos everglaculata and the frozen sections of adult Drosophila melanogaster were used as fluorescent antibody test (IFAT) and immunoenzyme staining test (IEST) to detect the sensitivity, specificity and cross-reactivity of adult worms and healthy adults. The sensitivity and specificity of the two antigens IFAT were 84.9-97.1% and 90.3-97.1% respectively; there was no cross-reaction to hookworm or roundworm infection. The sensitivity and specificity of the two antigens IEST were 94.3-94.9% and 97.1-100% respectively. It was more practical to use the frozen section antigen of adult worm filariasis: IEST was applied in the field.