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目的探讨乳腺上皮小黏蛋白(SBEM)和糖类抗原153(CA153)联合检测在乳腺癌微转移中的临床价值。方法选取2014年1月至2016年6月间上海市徐汇区大华医院收治的60例乳腺癌患者的血标本为观察组,选取20例乳腺纤维腺瘤患者、10例其他系统恶性肿瘤患者和10例健康志愿者的血标本为对照组。分别采用实时荧光定量PCR(RT-PCR)技术和化学发光法检测两组血标本中的SBEM mRNA及CA153的表达水平,并分析其在乳腺癌微转移中的诊断价值。结果RT-PCR检测显示观察组血标本中SBEM mRNA阳性表达率为48.3%(29/60),而对照组血标本的中SBEM mRNA阳性表达率为0.0%(0/40);CA153敏感度为31.7%(19/60),特异度为92.5%(37/40),两者联合检测敏感度为63.3%(38/60)。乳腺癌患者外周血SBEM mRNA表达与患者有无淋巴结转移和TNM分期差异有统计学意义(P<0.05),同时SBEM mRNA的表达与CA153的表达差异有统计学意义(P<0.05)。结论外周血SBEM mRNA和CA153联合检测提高了检测敏感度,有助于判断乳腺癌微转移。
Objective To investigate the clinical value of combined detection of SBEM and CA153 in breast cancer micrometastasis. Methods The blood samples from 60 cases of breast cancer admitted to Dahua Hospital of Xuhui District, Shanghai from January 2014 to June 2016 were selected as observation group. Twenty patients with breast fibroadenoma, 10 patients with other malignant tumors and Blood samples of 10 healthy volunteers were control group. The expression levels of SBEM mRNA and CA153 in blood samples were detected by real-time fluorescence quantitative PCR (RT-PCR) and chemiluminescence. The diagnostic value of SBEM mRNA and CA153 in breast cancer was analyzed. Results The positive rate of SBEM mRNA in the blood of the observation group was 48.3% (29/60), while the positive rate of SBEM mRNA in the blood of the control group was 0.0% (0/40). The sensitivity of CA153 was 31.7% (19/60), specificity was 92.5% (37/40), the combined detection sensitivity was 63.3% (38/60). The expression of SBEM mRNA in peripheral blood of patients with breast cancer was significantly higher than that in patients with or without lymph node metastasis and TNM stage (P <0.05). There was also a significant difference between SBEM mRNA expression and CA153 expression (P <0.05). Conclusion Combined detection of SBEM mRNA and CA153 in peripheral blood improves the detection sensitivity and helps to determine the micrometastasis of breast cancer.