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目的:建立痔裂敌颗粒中芦丁含量的HPLC测定方法。方法:色谱柱为Agilent TC ODS C18柱(5μm,4.6mm×250 mm);流动相为乙腈-0.1%冰醋酸溶液(pH3.16)(20∶80),流速1.0 ml/min,检测波长256 nm;柱温为35℃。结果:芦丁线性范围为0.032~0.65μg,平均回收率99.23%,RSD为0.45%。结论:该方法结果准确、重现性好,为控制痔裂敌颗粒的内在质量提供了科学依据。
Objective: To establish an HPLC method for determination of rutin in cleavable enemies. Method: The column was Agilent TC ODS C18 column (5μm, 4.6mm×250mm); the mobile phase was acetonitrile-0.1% glacial acetic acid solution (pH3.16) (20:80), the flow rate was 1.0 ml/min, and the detection wavelength was 256. Nm; column temperature is 35°C. Results: The linear range of rutin was 0.032 ~ 0.65μg, the average recovery rate was 99.23%, RSD was 0.45%. Conclusion: The method is accurate and reproducible. It provides a scientific basis for controlling the intrinsic quality of the cleave enemies.