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选Wistar系SPF/VAF雄性大鼠(n=32)均于7时～19时进行实验。①下丘脑室旁核(PNV)分大细胞群(PVN-mgn)和小细胞群(PVN-prv),植入刺激用慢性电极。手术后10日,运用于大鼠足蹠部施行的无侵袭性痛阈值测定法观察刺激的镇痛作用,并观察电刺激诱导的痛觉缺失(ESIA)。②探讨鸦片
All Wistar male SPF / VAF rats (n = 32) were tested at 7 o’clock to 19 o’clock. (1) The hypothalamic paraventricular nucleus (PNV) was divided into large cell group (PVN-mgn) and small cell group (PVN-prv), implanted with chronic electrode. Ten days after the operation, the analgesic effect of the stimulus was observed using a noninvasive threshold method for the determination of pain in the foot of the rat and electrical stimulation-induced pain loss (ESIA) was observed. ② explore opium