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目的探讨胎盘组织中色素上皮衍生因子(PEDF)和血管内皮生长因子(VEGF)的表达与子痫前期发病的关系。方法选择2011年10月至2012年10月于南方医科大学南方医院住院分娩的子痫前期患者36例为研究对象,其中轻度子痫前期(MPE)20例,重度子痫前期(SPE)16例。同期分娩的健康孕妇30例作为对照组。用免疫荧光组织化学双重标记方法检测所有研究对象胎盘组织中PEDF、VEGF的表达,并计数胎盘微血管密度(MVD),进行相关性分析。结果 (1)子痫前期患者胎盘组织中PEDF阳性表达明显高于正常对照组(P<0.05),SPE组PEDF阳性表达明显高于MPE组(P<0.05)。(2)与正常对照组相比,子痫前期患者胎盘组织中VEGF表达减少(P<0.05),MPE与SPE组VEGF表达差异无统计学意义(P>0.05)。(3)正常对照组、MPE组、SPE组MVD依次递减(P<0.05)。(4)子痫前期组和正常对照组胎盘中PEDF表达与VEGF表达及MVD呈负相关(r=-0.272,P<0.05;r=-0.418,P<0.01),VEGF表达与MVD数值的变化则呈正相关(r=0.443,P<0.05)。结论 PEDF与VEGF在胎盘的滋养层细胞和血管内皮细胞中表达位置大体相同。子痫前期患者胎盘组织中PEDF表达升高,而VEGF表达及MVD下降,后两者均与PEDF表达呈负相关,可能与子痫前期的发病及病情严重程度相关。
Objective To investigate the relationship between the expression of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in placenta and the incidence of preeclampsia. Methods Thirty-six patients with preeclampsia who were hospitalized and delivered in Nanfang Hospital of Southern Medical University from October 2011 to October 2012 were selected as the study subjects, including 20 mild preeclampsia (MPE) and 16 severe preeclampsia (SPE) example. The same period of delivery of healthy pregnant women 30 cases as a control group. Immunofluorescence histochemical double labeling method was used to detect the expression of PEDF and VEGF in placenta of all subjects and the placental microvessel density (MVD) was counted for correlation analysis. Results (1) The positive expression of PEDF in placenta of preeclampsia patients was significantly higher than that in normal controls (P <0.05). The positive expression of PEDF in SPE group was significantly higher than that of MPE group (P <0.05). (2) Compared with the normal control group, the expression of VEGF in placenta of preeclampsia patients decreased (P <0.05), but there was no significant difference between MPE and SPE groups (P> 0.05). (3) MVD in normal control group, MPE group and SPE group decreased in turn (P <0.05). (4) The expression of PEDF in placentas of preeclampsia group and normal control group was negatively correlated with the expression of VEGF and MVD (r = -0.272, P <0.05; r = -0.418, P <0.01) There was a positive correlation (r = 0.443, P <0.05). Conclusion The expression of PEDF and VEGF in placental trophoblastic cells and vascular endothelial cells in the same position. The expression of PEDF in placenta of preeclampsia patients increased, while the expression of VEGF and MVD decreased, both of which were negatively correlated with the expression of PEDF, which may be related to the incidence of preeclampsia and the severity of the disease.