茚三酮显色法定性定量测定氨基酸是一种常用的氨基酸测定方法。这里介绍一种经改进后的茚三酮显色法,进行微量氨基酸定性测定。一、试剂配制: 1.0.01M氰化钾:称0.1628克氰化钾,溶解于少量蒸馏水中,定容量至250毫升。 2.氰化钾—乙二醇甲醚—茚三酮显色剂:称105克茚三酮,溶解于25毫升乙二醇 Ninhydrin colorimetric qualitative and quantitative determination of amino acids is a commonly used amino acid determination method. Here introduced a modified ninhydrin chromogenic method for the qualitative determination of trace amino acids. First, reagent preparation: 1.0.01M Potassium cyanide: Weigh 0.1628 grams of potassium cyanide, dissolved in a small amount of distilled water, constant volume to 250 ml. 2. Potassium cyanide - ethylene glycol methyl ether - ninhydrin developer: 105 g of ninhydrin, said dissolved in 25 ml of ethylene glycol