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目的： 观察蒿甲醚（ Ａｒｔ） 对日本血吸虫核苷摄入和核酸含量的影响。方法： 感染小鼠１ 次灌服（ｉｇ） Ａｒｔ ３００ ｍｇ／ｋｇ 后２４ ｈ 或４８ ｈ ， 分别测定雌虫（ ♀） 、雄虫（ ♂） 的 Ｒ Ｎ Ａ 和 Ｄ Ｎ Ａ 含量。另取经 Ａｒｔ 作用２４ ｈ 后的♀及♂虫体， 作体外培养２ ｈ 或４ ｈ ， 测定虫体对３ Ｈ 腺苷、［５３ Ｈ］ 尿苷和［ 甲基３ Ｈ］ 胸苷的摄入量以及上述３ Ｈ 标记核苷摄入虫体核酸的量。结果： 经 Ａｒｔ 体内作用４８ ｈ 后， 血吸虫♀虫的 Ｒ Ｎ Ａ 和 Ｄ Ｎ Ａ 含量分别较对照组减少５１６ ％ 和２３５ ％ ， 差异均显著， 而♂虫 Ｒ Ｎ Ａ 含量减少４２４ ％ 。♀及♂虫体经 Ａｒｔ 作用后移置体外培养２ｈ 或４ ｈ ， ♀虫对３ 种３ Ｈ 标记核苷的摄入量均较对照组的明显为少， 减少率达３５２ ％ ～５０１ ％ 。体外培养２ ｈ ，［ 甲基３ Ｈ］ 胸苷掺入♀虫 Ｄ Ｎ Ａ 的量较对照组减少７１４ ％ ， 培养４ ｈ 后， ［３ Ｈ］ 腺苷掺入♀虫 Ｒ Ｎ Ａ 和 Ｄ Ｎ Ａ 的量较对照组减少６５２ ％ 与５００ ％ 。结论： Ａｒｔ 对日本血吸虫， 尤其是♀虫的核酸代谢有抑制作用。
Objective: To observe the effect of Artemether on the nucleotide uptake and nucleic acid content of Schistosoma japonicum. Methods: The contents of R N A and D N A in male and female adults (♂ and ♂) were measured 24 h or 48 h after infection with Art 300 mg / kg once. In addition, Artemisia sp. And ♂ parasites after 24 h of Art treatment were cultured for 2 h or 4 h in vitro, and the activities of 3 H-adenosine, 5-H] uridine and [3 H] thymidine And the amount of nucleic acid ingested by the 3 H-labeled nucleosides. Results: The contents of R N A and D N A were significantly decreased by 516% and 235%, respectively, compared with those in the control group after 48 h of Art in vivo. However, the content of R N A decreased by 42% 4%. ♀ and ♂ parasites Art after transplantation in vitro cultured 2h or 4 h, pinworm on the 3 kinds of 3H labeled nucleoside intake were significantly less than the control group, a reduction rate of 35 2% ~ 50 1%. After cultured for 2 h in vitro, the amount of D N A bound to [ 3H] thymidine was reduced by 714% compared with that of the control group. After 4 h incubation, the [H] And D N A decreased by 652% and 500% compared with the control group. Conclusion: Art has an inhibitory effect on the nucleic acid metabolism of Schistosoma japonicum, especially pinworm.