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目的: 观察蒿甲醚( Art) 对日本血吸虫核苷摄入和核酸含量的影响。方法: 感染小鼠1 次灌服(ig) Art 300 mg/kg 后24 h 或48 h , 分别测定雌虫( ♀) 、雄虫( ♂) 的 R N A 和 D N A 含量。另取经 Art 作用24 h 后的♀及♂虫体, 作体外培养2 h 或4 h , 测定虫体对3 H 腺苷、[53 H] 尿苷和[ 甲基3 H] 胸苷的摄入量以及上述3 H 标记核苷摄入虫体核酸的量。结果: 经 Art 体内作用48 h 后, 血吸虫♀虫的 R N A 和 D N A 含量分别较对照组减少516 % 和235 % , 差异均显著, 而♂虫 R N A 含量减少424 % 。♀及♂虫体经 Art 作用后移置体外培养2h 或4 h , ♀虫对3 种3 H 标记核苷的摄入量均较对照组的明显为少, 减少率达352 % ~501 % 。体外培养2 h ,[ 甲基3 H] 胸苷掺入♀虫 D N A 的量较对照组减少714 % , 培养4 h 后, [3 H] 腺苷掺入♀虫 R N A 和 D N A 的量较对照组减少652 % 与500 % 。结论: Art 对日本血吸虫, 尤其是♀虫的核酸代谢有抑制作用。
Objective: To observe the effect of Artemether on the nucleotide uptake and nucleic acid content of Schistosoma japonicum. Methods: The contents of R N A and D N A in male and female adults (♂ and ♂) were measured 24 h or 48 h after infection with Art 300 mg / kg once. In addition, Artemisia sp. And ♂ parasites after 24 h of Art treatment were cultured for 2 h or 4 h in vitro, and the activities of 3 H-adenosine, 5-H] uridine and [3 H] thymidine And the amount of nucleic acid ingested by the 3 H-labeled nucleosides. Results: The contents of R N A and D N A were significantly decreased by 516% and 235%, respectively, compared with those in the control group after 48 h of Art in vivo. However, the content of R N A decreased by 42% 4%. ♀ and ♂ parasites Art after transplantation in vitro cultured 2h or 4 h, pinworm on the 3 kinds of 3H labeled nucleoside intake were significantly less than the control group, a reduction rate of 35 2% ~ 50 1%. After cultured for 2 h in vitro, the amount of D N A bound to [ 3H] thymidine was reduced by 714% compared with that of the control group. After 4 h incubation, the [H] And D N A decreased by 652% and 500% compared with the control group. Conclusion: Art has an inhibitory effect on the nucleic acid metabolism of Schistosoma japonicum, especially pinworm.