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利用开顶箱熏蒸法,研究了高浓度O3(≈80nmol·mol-1)和高浓度CO2(≈700μmol·mol-1)及其复合处理对蒙古栎叶片活性氧代谢的影响.结果表明:高浓度O3显著增加了蒙古栎叶片超氧阴离子(O2·-)产生速率、过氧化氢(H2O2)和丙二醛(MDA)含量和电解质外渗率(P<0.05),显著降低了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性和抗坏血酸(AsA)含量(P<0.05).高浓度CO2对蒙古栎叶片活性氧代谢影响不显著.高浓度O3和CO2复合处理的叶片O2.-产生速率、H2O2和MDA含量和电解质外渗率上升不明显,说明高浓度CO2缓解了高浓度O3对蒙古栎叶片的氧化胁迫.复合处理的叶片SOD、CAT、APX活性以及AsA和总酚含量显著高于O3处理的叶片(P<0.05),说明高浓度CO2缓解了高浓度O3对叶片抗氧化系统的消极影响.
The effects of high concentrations of O3 (≈80nmol · mol-1) and high concentrations of CO2 (≈700μmol · mol-1) and their combined treatments on active oxygen metabolism in Quercus mongolica leaves were studied by open top box fumigation method. The results showed that high O3 concentration significantly increased the O2 · - production rate, hydrogen peroxide (H2O2), malondialdehyde (MDA) content and electrolyte leakage rate (P0.05), significantly decreased the content of superoxide anion SOD, CAT, APX and AsA contents (P <0.05) .High concentration of CO2 had no significant effect on the active oxygen metabolism of Quercus mongolica leaves. O 2 -production rate, H 2 O 2 and MDA contents and electrolyte leakage rate did not increase significantly with O 3 -concentration CO 2 treatment, indicating that high concentration of CO 2 could alleviate the oxidative stress on the leaves of Quercus mongolica by high concentrations of O3.The leaf SOD, CAT, APX activity and AsA and total phenolic content were significantly higher than that of O3 treatment (P <0.05), indicating that high concentration of CO2 relieved the negative effect of high concentration of O3 on leaf antioxidant system.