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利用原子力显微镜分别对未加药人急性T淋巴细胞性白血病细胞(Jurkat细胞)和经超抗原刺激不同时间(6,12,48,72h)后的Jurkat细胞进行了细胞全貌和细胞膜表面纳米结构成像和探测研究,并通过比较不同状态下细胞表面的粘附力变化,探讨Jurkat细胞形态变化与粘附行为之间的关系,用CCK-8检测细胞的增殖,以期对Jurkat细胞形态结构和细胞功能之间的关系有进一步的了解。结果发现:与未加药的Jurkat细胞相比,随着超抗原刺激时间的延长,Jurkat细胞的体积、高度、半宽度、粗糙度等参数发生明显的变化;活化48、72h时,细胞与针尖间的相互作用力大约是活化6h时的5倍,活化过程中细胞膜表面纳米结构的改变,引起其机械性能的变化。Jurkat细胞的表面超微结构、细胞膜结构的改变和分化对于更深入地了解T细胞活化与免疫信号的传递,阐明其免疫过程的作用机制具有重要的意义。
Atomic force microscopy was performed on intact human Jurkat cells and Jurkat cells stimulated with superantigen at different time points (6, 12, 48 and 72 hours) respectively. Cell morphology and cell surface nanostructure imaging And explore the relationship between Jurkat cell morphological changes and adhesion behavior by comparing the changes of cell surface adhesion under different conditions, CCK-8 detection of cell proliferation in order to Jurkat cell morphology and cell function The relationship between the further understanding. The results showed that compared with the untreated Jurkat cells, the parameters of Jurkat cells such as volume, height, half-width, roughness and so on changed obviously with the prolongation of superantigen stimulation time. When activated for 48 and 72h, Interaction between the activation of about 6h 5 times the activation of the cell membrane surface nanostructures change, causing changes in their mechanical properties. The ultrastructure of Jurkat cells, the alteration and differentiation of cell membrane structure are of great significance for understanding the mechanism of T cell activation and immune signal transmission and elucidating the immune process.