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选用与ＨｕＩＦＮ－α１基因及其信号肽相应的引物，采用ＰＣＲ技术，从中国人血白细胞染色体中分离带有信号肽ＩＦＮ－α１基因片段，分别克隆到质粒Ｍ１３ｍｐ１９和Ｍ１３ｍｐ１８中，通过序列分析进行鉴定，然后克隆到家蚕多角体病毒（１３０ｍｂｙｘｍｏｒｉＮｕｃｌｅａｒＰｏｌｙｈｅｄｒｏｓｉｓＶｉｒｕｓ，ＢｍＮＰＶ）载体ＰＢＦ５的ＥｃｏＲＩ和ＸｂａＩ之间，用合成的相应寡核苷酸片段为引物进行双链测序，鉴定为阳性重组转移载体后，将其ＤＮＡ和ＢｍＮＰＶ基因组ＤＮＡ共转染家蚕细胞，筛选出重组病毒，再将其感染家蚕细胞，测得细胞培养上清中ＩＦＮ活性为１．０×１０６ＩＵ／ｍｌ。
The gene fragment of IFN-α1 with signal peptide was isolated from chromosomes of Chinese white blood cells by PCR using primers corresponding to HuIFN-α1 gene and its signal peptide, and cloned into plasmids M13mp19 and M13mp18, respectively, and identified by sequence analysis , Then cloned into EcoRI and XbaI of the PBM5 vector of Bombyx mori Nucleopolyhedrovirus (BmNPV), double-stranded sequencing was performed using the corresponding oligonucleotide fragments as primers, and after being identified as a positive recombinant transfer vector, the DNA and BmNPV genome DNA co-transfected silkworm cells, screened out recombinant virus, and then infected with silkworm cells, measured in cell culture supernatant IFN activity was 1.0 × 106IU / ml.