论文部分内容阅读
目的 研究表达狂犬病毒 3aG株糖蛋白 (GP)的重组复制缺陷型腺病毒Ad/GP′及Ad/GP免疫小鼠后所产生的特异性体液免疫应答 ,体外脾细胞增殖反应 ,及免疫小鼠对狂犬病毒致死性颅内攻击的保护力。方法 1× 10 7pfu重组病毒经腹腔对小鼠进行基础和加强免疫 ,以快速荧光灶抑制实验 (RFFIT)方法测定小鼠血清狂犬病毒特异性中和抗体滴度 ,[3H]TdR掺入DNA法测定体外脾细胞增殖反应 ;小鼠致死性CVS狂犬病毒颅内攻击测定重组病毒免疫对小鼠的保护效果。结果 RFFIT方法测定免疫小鼠血清中和抗体滴度分别为Ad/GP :0 75IU/ml,Ad/GP’ :2 6IU/ml;[3H]TdR掺入法测定重组病毒免疫组小鼠脾细胞体外受到特异GP抗原刺激后 ,增殖增强 2倍以上 ;86 7%的Ad/GP′免疫小鼠及 6 6 7%的Ad/GP免疫小鼠可抵抗约 30LD50 CVS狂犬病毒的颅内攻击。结论 表达狂犬病毒G蛋白的重组复制缺陷型腺病毒具有用做基因工程重组病毒载体活疫苗的好前景
Objective To study the specific humoral immune response, the splenocyte proliferation response in vitro and the immunization of mice immunized with recombinant replication-defective adenovirus Ad / GP ’expressing rabies virus 3aG glycoprotein (GP) Rabies virus lethal intracranial attack protection. Methods The mice were immunized by 1 × 10 7pfu recombinant virus via intraperitoneal injection. The rabies virus specific neutralizing antibody titers were determined by RFFIT method and the incorporation of [3H] TdR into DNA To determine the proliferative response of splenocytes in vitro; To determine the protective effect of recombinant virus on mice by lethal CVS rabies virus in mice. Results The titer of neutralizing antibody in the immunized mice was determined by RFFIT method. Ad / GP: 0.75 IU / ml and Ad / GP ’: 26 IU / ml, respectively. In vitro stimulated by specific GP antigen, the proliferation increased more than 2 times; 86 7% of Ad / GP ’immunized mice and 6 6 7% of Ad / GP immunized mice were resistant to 30LD50 CVS rabies virus intracranial attack. Conclusions Recombinant replication-defective adenovirus expressing rabies virus G protein has good prospects for use as a live-in vaccine for genetically engineered recombinant viral vectors