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目前利用培养细胞进行的研究工作日益增多,特别是肿瘤细胞的体外培养或建株更受到重视。建国以来,我国自建了不少人肿瘤细胞系,而动物肿瘤细胞系却比较少。H615是本院病理生理教研室建立的国内第一株可移植性小鼠肝细胞性肝癌模型,为了观察H615体外培养的特性,摸索其生长条件,并进一步建立H615细胞系供实验研究用,我们从1980年10月起,用多种方法对H615进行了原代培
At present, the research work using cultured cells is increasing, especially the in vitro culture or establishment of tumor cells. Since the founding of the People’s Republic of China, many human cancer cell lines have been established in our country, but there are fewer animal tumor cell lines. H615 is the first transplantable mouse hepatocellular carcinoma model established in our hospital’s Department of Pathophysiology, in order to observe the characteristics of H615 culture in vitro, explore its growth conditions, and further establish the H615 cell line for experimental research. From October 1980, the H 615 was subjected to primary culture in various ways.