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将酶联聚合反应（ＰＣＲ）法在检测各种镰刀菌种类和Ｍｉｃｒｏｄｏｃｂｉｕｍｎｉｖａｌｅ小种与用于田间小区（中间副区为Ｆ．ｃｕｌｍｏｒｕｍ接种区）小麦传统的病害观察评价进行比较。在ＧＳ８０时从１５个副区中分别采集一定样品进行病害观察评价。收获时，将每个样品分成籽粒、颖片和穗轴三个部分，再用ＰＣＲ分析检测Ｆ．ｃｕｌｍｏｒｕｍ、Ｆ．ｐｏａｅ、Ｆ．ｇｒａｍｉｎｅａｒｕｍ、Ｍ．ｎｉｖａｌｅｖａｒ．ｍａｊｕｓ和Ｍ．ｎｉｖａｌｅｖａｒ．ｎｉｖａｌｅ．在接种区，病害观察评价与ＰＣＲ相关良好，两种方法均表明这个区域的Ｆ．ｃｕｌｍｏｒｕｍ的高指数。根据观察结果，在田间小区的其它副区中大多数有相对高的Ｆ．ｃｕｌｍｏｒｕｍ指数。然而，依据ＰＣＲ分析，Ｆ．ｃｕｌｍｏｒｕｍ指数在许多其它的小区相对很低，且在这些小区的许多样穗的籽粒颖片和穗轴中检测到Ｆ．ｐｏａｅ、Ｍ．ｎｉ－ｖａｌｅｖａｒ．ｍａｊｕｓ和ｖａｒ．ｎｉｖａｌｅ以及Ｆ．ａｖｅｎａｃｕｍ。Ｆ．ｐｏａｅ在颖片中占优势，而Ｍｎｉｖａｌｅｖａｒ．ｍａｉｕｓ和ｖａｒ．ｎｉｖａｌｅ在穗轴中占优势。Ｍ．ｎｉｖａｌｅ的ＰＣＲ分析结果表明，６４％的病穗与ｖａｒ．ｍａｉｕｓ有关，３６％的与ｖａｒ．ｎ?
The enzyme-linked polymerase chain reaction (PCR) method was used to compare the evaluation of traditional disease detection of various Fusarium species and Microdocbium nivale races with wheat for the field plot (F.culmorum inoculation). At GS80, 15 samples from 15 sub-zones were collected for disease observation and evaluation. At the time of harvest, each sample was divided into three parts: grain, glume and cob, and PCR was performed to detect F culmorum, F poae, F. graminearum, M. nivalevar. majus and M. nivalevar. nivale In the inoculation zone, the disease observation and evaluation were well correlated with PCR, both of which showed F in this area. culmorum high index. According to the observation, most of the other sub-zones in the field have a relatively high F. culmorum index. However, based on PCR analysis, F. The culmorum index is relatively low in many other plots and F was detected in many panicle glumes and cob in these plots. poae, M. ni-valevar. majus and var. nivale and f avenacum. F. Poae dominant in glumes, while Mnivalevar. maius and var. nivale is dominant in cob. M. PCR analysis of nivale showed that 64% of diseased spikes were associated with var. About maius, 36% with var. n?