论文部分内容阅读
目的将奖励性操作式条件反射方法应用于学习记忆研究。方法首次采用奖励性操作式条件反射方法,设置单次操作训练、连续多次操作训练、信号辨识与消退四种检测模式,研究Wistar大鼠和SHR大鼠学习记忆能力。结果奖赏训练中,SHR组鼻触次数显著增多(vs.Wistar&Donepezil,P<0.05);单次操作训练中,三组动物对操作反应的获得无显著差异;连续多次操作学习任务中,SHR组错误操作次数增多(vs.Wistar,P<0.05),奖赏获得次数减少(vs.Donepezil,P<0.001),反应准确率显著降低(vs.Wistar&Donepezil,P<0.05);信号辨识任务中SHR组错误反应次数显著增多(vs.Wistar&Donepezil,P<0.05),而反应准确率降低,组间差异有显著性(vs.Wistar&Donepezil,P<0.05);消退实验中,三组动物差异无显著性。神经递质检测发现,SHR组大鼠谷氨酸[(1.0639±0.07086)mg/g]、乙酰胆碱[(2.7760±0.2609)μg/g]与五羟色胺[(1.2200±0.1137)μg/g]含量均显著低于Wistar组大鼠(P<0.05),多奈哌齐组大鼠乙酰胆碱含量显著增加[(3.9344±0.2747)μg/g](vs.Wistar,P<0.05;vs.SHR,P<0.001)。结论奖励性操作式条件反射方法可作为一种正性增强条件反射检测新方法应用于大鼠学习记忆研究。
Objective To apply rewards-operated conditional reflex method to learning and memory research. Methods For the first time, rewards-based conditional reflex method was used to set up learning and memory abilities of Wistar rats and SHR rats by setting one-time operation training, continuous operation training, signal identification and extinction. In the reward rewards training, the number of nasal touches significantly increased in SHR group (vs. Wistar & Donepezil, P <0.05). In the single-operation training, there were no significant differences among the three groups in obtaining operational response; in the continuous multi-task learning task, SHR group (W vs. Wistar & Donepezil, P <0.05); error of SHR group in signal identification task (Wistar, P <0.05) (Vs. Wistar & Donepezil, P <0.05), and the accuracy of response decreased with a significant difference between groups (vs. Wistar & Donepezil, P <0.05). There was no significant difference in the regression test between the three groups of animals. The results of neurotransmitters showed that the contents of glutamate ([(1.0639 ± 0.07086) mg / g], acetylcholine [(2.7760 ± 0.2609) μg / g] and serotonin in SHR group [(1.2200 ± 0.1137) μg / g] The levels of acetylcholine in the donepezil group were significantly higher than those in the Wistar group [(3.9344 ± 0.2747) μg / g vs Wistar, P <0.05 vs. SHR, P <0.001). CONCLUSION: Reward-based manipulative conditional reflex method can be used as a positive enhancement of conditional reflex detection in the study of learning and memory in rats.