目的　探讨金属蛋白酶组织抑制因子 (TIMP 2 )对人脑胶质瘤细胞的抑制作用 ,主要是对细胞周期与细胞增殖的作用。方法　用含TIMP 2基因的重组腺病毒载体 ,体外转染人胶质瘤细胞系U87,用Westernblot检测目的蛋白的表达。通过细胞生长实验 ,流式细胞仪分析技术检测U87转染前后细胞增殖、周期与凋亡的变化。结果　转染AdTIMP 2病毒后的U87细胞TIMP 2蛋白表达上调 ,转染后对U87细胞的生长有抑制作用 ,并出现明显的G0 G1期阻滞 ,但无明显的细胞凋亡峰出现。结论　重组腺病毒载体介导的TIMP 2基因在体外对人脑胶质瘤细胞系U87的生长有抑制作用 ,可作为胶质瘤治疗的有效工具 Objective To investigate the inhibitory effect of tissue inhibitor of metalloproteinase (TIMP 2) on human glioma cells, mainly on cell cycle and cell proliferation. Methods The human glioma cell line U87 was transfected with the recombinant adenovirus vector containing TIMP2 gene and the expression of the target protein was detected by Western blot. Cell proliferation, cell cycle and apoptosis were detected by flow cytometry and flow cytometry before and after U87 transfection. Results The expression of TIMP-2 protein was up-regulated in U87 cells transfected with AdTIMP 2 virus. The transfection of UTI inhibited the growth of U87 cells and markedly blocked G0 G1 phase. However, no obvious apoptotic peak appeared. Conclusions The recombinant adenovirus vector-mediated TIMP-2 gene can inhibit the growth of human glioma cell line U87 in vitro and can be used as an effective tool for the treatment of glioma