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目的探讨CXCR4-shRNA腺病毒载体对人胃癌细胞裸鼠腹腔转移瘤的抑制作用。方法构建裸鼠腹腔转移瘤模型,分为SGC7901组、空病毒组、CXCR4-shRNA组,比较3组裸鼠肿瘤体积、重量,转移灶肿瘤数,裸鼠生存时间、生存延长率、抑瘤率。Western blot检测肿瘤组织VEGF-C、MPP-2蛋白的表达情况。结果接种8～15d后裸鼠均有肿瘤长出,术区可触及大小2～3cm的结节,质硬且活动不良。shRNA组肿瘤体积平均(0.78±0.14)mm3、重量平均(1.20±0.16)g、数目平均(5.75±1.39)个,与SGC7901组[(5.61±0.44)mm3、(3.93±0.19)g、(28.86±1.83)个]及空病毒组[(4.65±0.42)mm3、(3.82±0.14)g、(27.75±1.39)个]比较差异有统计学意义(P﹤0.01);SGC7901组及空病毒组比较,差异无统计学意义(P﹥0.05)。SGC7901组生存时间平均(25.50±1.28)d,空病毒组(29.00±1.60)d,CXCR4-shRNA组(40.62±2.06)d;CXCR4-shRNA组生存延长率及抑瘤率分别为59.3%及69.5%,空病毒组分别为13.7%及2.8%。Westernblot检测结果显示,CXCR4-shRNA组VEGF-C、MMP-2蛋白表达水平明显弱于SGC7901组及空病毒组。结论 CXCR4-shRNA腺病毒载体可抑制人胃癌细胞裸鼠腹腔转移瘤的生长及转移。
Objective To investigate the inhibitory effect of CXCR4-shRNA adenovirus vector on human gastric cancer cells in nude mice peritoneal metastases. Methods The model of peritoneal metastasis in nude mice was established and divided into SGC7901 group, empty virus group and CXCR4-shRNA group. The tumor volume, weight, number of tumor in metastatic tumor, survival time in nude mice, survival extension rate, tumor inhibition rate . Western blot detection of VEGF-C, MPP-2 protein expression. Results All the nude mice grew 8 to 15 days after inoculation, and the nodules of 2 ~ 3 cm in size could be touched in the operation area, which were hard and not active. The tumor volume of the shRNA group was (0.78 ± 0.14) mm3, and the average weight was (1.20 ± 0.16) g, the average number was 5.75 ± 1.39. Compared with the SGC7901 group, the tumor volume of the shRNA group was (5.61 ± 0.44) mm3, (3.93 ± 0.19) g (1.65 ± 0.42) mm3, (3.82 ± 0.14) g, (27.75 ± 1.39), respectively] (P <0.01). There were significant differences between SGC7901 group and empty virus group , The difference was not statistically significant (P> 0.05). The survival time of SGC7901 group was (25.50 ± 1.28) d, empty virus group (29.00 ± 1.60) d and CXCR4-shRNA group (40.62 ± 2.06) d respectively. The survival and anti-tumor rates of CXCR4-shRNA group were 59.3% and 69.5 %, Empty virus group were 13.7% and 2.8% respectively. Western blot results showed that the expression of VEGF-C and MMP-2 in CXCR4-shRNA group was significantly weaker than that in SGC7901 group and empty virus group. Conclusion CXCR4-shRNA adenovirus vector can inhibit the growth and metastasis of human gastric cancer cells in nude mice peritoneal metastases.