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目的:探讨癌性营养不良小鼠肠黏膜损伤与炎症反应的关系。方法:将14只小鼠随机分为对照组和荷瘤组。荷瘤组皮下种植小鼠结肠腺癌26细胞悬液(1×106个/只)。接种后检测两组小鼠的体质量变化。第16天测量小肠黏膜的绒毛高度、绒毛宽度、基底层厚度和肌层厚度,检测血清和小肠组织中肿瘤坏死因子-α(TNF-α)、IL-1、IL-6、IL-10、γ干扰素(IFN-γ)、分泌型肿瘤坏死因子受体Ⅰ(sTNF-RⅠ)和sTNF-RⅡ的量。结果:实验第16天荷瘤组小鼠去瘤体质量、小肠绒毛高度、基底层厚度明显低于对照组,有显著性差异(P<0.05)。绒毛宽度、肌层厚度无明显改变。荷瘤组小鼠血清中TNF-α、IL-6、sTNF-RⅠ浓度高于对照组,IL-10浓度则低于对照组。荷瘤组小鼠小肠组织中TNF-α浓度高于对照组,而IL-1、IFN-γ、sTNF-RⅠ和sTNF-RⅡ浓度均低于对照组。结论:癌性恶病质小鼠存在肠黏膜损伤,血清和肠组织中存在广泛的细胞因子浸润,两者可能有相关性。
Objective: To investigate the relationship between intestinal mucosal lesion and inflammatory reaction in mice with cancerous dystrophy. Methods: 14 mice were randomly divided into control group and tumor-bearing group. Tumor-bearing mice were subcutaneously colonized with 26 colon adenocarcinoma cells (1 × 106 cells / mouse). After inoculation, the body weight of two groups of mice was tested. On the 16th day, the villus height, villi width, basilar layer thickness and muscular layer thickness of intestinal mucosa were measured to detect the levels of tumor necrosis factor-α (TNF-α), IL-1, IL-6 and IL- γ interferon (IFN-γ), secreted tumor necrosis factor receptor Ⅰ (sTNF-RI), and sTNF-RII. Results: On the 16th day in the experiment, the tumor weight, the villus height and the thickness of the basilar layer in the tumor-bearing mice were significantly lower than those in the control group (P <0.05). Villus width, muscle thickness no significant change. The serum concentrations of TNF-α, IL-6 and sTNF-RⅠ in the tumor-bearing group were higher than those in the control group, but the concentrations of IL-10 in the tumor-bearing group were lower than those in the control group. The concentration of TNF-α in the small intestine of the tumor-bearing mice was higher than that of the control mice, while the concentrations of IL-1, IFN-γ, sTNF-RⅠ and sTNF-RⅡ were lower than those of the control group. CONCLUSION: Mice with malignant cachexia have intestinal mucosal injury and extensive cytokine infiltration in serum and intestinal tissue, which may be related to each other.