论文部分内容阅读
目的采用大肠埃希菌O157∶H7-EDL933株,实验感染家蝇,观察家蝇体内外感染和带菌消长情况。方法点滴法体外定量染菌,剂量为1μ(l菌悬液浓度为8.0×105～1.9×107cfu/ml),并采用昆虫单虫单管方法饲养观察。结果家蝇经体外染菌,获得体内外带菌,体外带菌时间中位数为(96.0±15.3)h(95%CI:66.0～126.0h);体内中位数为(144.0±17.3)h(95%CI:110.2～177.8h);家蝇体内外带菌消长差异无统计学意义(χ2=0.74,P=0.388),家蝇最长带菌时间至少216h(9d)。结论研究结果提示,家蝇不仅是O157∶H7疾病传播的媒介,更重要的可能是人类感染潜在的贮存宿主。
Objective To investigate the in vivo and in vitro infection and carriage of housefly in housefly with Escherichia coli O157: H7-EDL933 strain. Methods Quantitative in vitro bacterial injections were carried out by spotting at a dose of 1μL (concentration of 8.0 × 105 ~ 1.9 × 107cfu / ml), and the single insect was used for feeding. Results The median length of carriage in vitro was (96.0 ± 15.3) h (95% CI: 66.0 ~ 126.0 h) and the median in vivo was (144.0 ± 17.3) h % CI: 110.2 ~ 177.8h). There was no significant difference in the growth and elimination of housefly in vivo and in vitro (χ2 = 0.74, P = 0.388), and the longest carriage time of housefly was at least 216h (9d). Conclusions The results suggest that housefly is not only a vector for the transmission of O157: H7 disease, but more importantly may be a potential reservoir for human infection.