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伤寒噬菌体分型在伤寒流行病学调查中,追索传染源,分析传播途径,判断流行菌株是本地或外来菌株等均有重要意义。为此,1985年底,就全省1981年以来搜集的397株伤寒杆菌进行噬菌体型别鉴定,并对安顺市1985年暴发流行中分离的84株伤寒杆菌作分型,现将结果报告如下: 材料和方法一.伤寒菌株:397株伤寒沙门氏菌,分别来自全省9个专州(市)23个县。全部菌株经初步生化(双糖)及玻片血清凝集鉴定(部分菌株作了系统生化试验),符合沙门氏菌特征。其“Vi”抗原经玻片凝集,强度均在“++”以上, 二.噬菌体:采用贵阳市防疫站从北京市防疫站引进的96型Vi噬菌体及相应菌种,自行增殖制备。
Typhoid bacteriophage type in epidemiological investigation of typhoid fever, recourse to the source of infection, analysis of transmission routes, to determine the prevalence of strains are local or foreign strains are of great significance. To this end, the end of 1985, the province collected since 1981, 397 strains of typhoid bacillus phage-type identification, and outbreak in 1985, Anshun outbreak of 84 strains of typhoid bacillus for the type, the results are reported as follows: Materials And method 1. Typhoid strains: 397 strains of Salmonella typhi, respectively, from the province’s nine prefectures (cities) 23 counties. All strains were identified by initial biochemical (disaccharide) and serum albumin agglutination (some of the strains were biochemically tested) in line with Salmonella characteristics. The “Vi” antigen agglutination by the slide, the intensity are above the “+ +”. II phage: Guiyang City epidemic prevention station from the Beijing Municipal Epidemic Prevention Station 96 type Vi phage and the corresponding species, self-proliferation preparation.