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目的探讨老龄大鼠脑内M胆碱受体和β肾上腺素受体的变化规律，以及不同补肾中药的调整作用。方法以3H－QNB和3H－DHA为标记配基，分别对M胆碱受体和β肾上腺素受体作放射配基结合分析。采用单点法测定受体数（RT）。结果早期衰老大鼠（22～26月龄）脑M受体的RT值降低约8％，滋阴药知母生地上调M受体的作用有显著意义，β受体的密度无显著变化，5种中药配方也均无明显作用；原期衰老大鼠（30～32月龄）脑内M受体RT值降低幅度约为10％左右；β受体RT值则显著大幅度下降，平均降低20％以上，补气药黄芪在上调M受体的同时，也明显上调β受体，其它4种中药对M和β受体作用均不显著。结论衰老早期以M受体下降为主，滋阴药知母生地有效康老晚期除M受体继续降低外，β受体降低幅度更大，补气药黄芪有效。
Objective To investigate the changes of M choline receptor and β-adrenergic receptor in the brain of aging rats and the regulatory effect of different Chinese herbs for tonifying kidney. Methods 3H-DNB and 3H-DHA were used as labeled ligands, and radioligand binding assay was performed on the M choline receptor and the β-adrenoceptor respectively. Receptor numbers (RT) were determined by single-point method. Results The RT value of M receptor in early aged rats (22 ~ 26 months old) decreased about 8%. The effect of up-regulating M receptor in Ziyin drug was significant, while the density of β receptor had no significant change. 5 There was no significant effect on the formulation of traditional Chinese medicine. In the aged rats (30-32 months old), the decrease of M receptor RT value in brain was about 10%, while the RT value of β receptor decreased significantly with an average decrease of 20 %, Astragalus mongholicus upregulation of M receptor, but also significantly upregulation of beta receptors, the other four kinds of Chinese medicine on M and beta receptors were not significant. Conclusion M receptor decreased mainly in the early stage of senescence, and the effect of nourishing yin drug was better than that of M receptor.