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目的 为有效提高小分子多肽在体诱导CTL应答的能力 ,制备了乙肝治疗性多肽免疫刺激复合物 (ISCOMs) ,并对其形成进行了鉴定。方法 乙肝治疗性多肽ISCOMs采用透析法制备获得 ,并经小分子多肽的SDS PAGE电泳法确定了复合物中小分子多肽的存在 ,采用Bradfords法测定了复合物中多肽的结合量。结果 在电镜下呈典型的蜂巢状亚微型颗粒结构 ,所制备的ISCOMs中多肽的结合量约为 0 .30mg/ml。结论 ISCOMs的成功制备 ,为进一步研究小分子多肽免疫刺激复合物的免疫学特性奠定了基础
Objective To improve the ability of small molecule peptides to induce CTL responses in vivo, Hepatitis B therapeutic polypeptide immunostimulating complexes (ISCOMs) were prepared and their formation was identified. Methods Hepatitis B therapeutic peptide ISCOMs was prepared by dialysis method. The presence of small molecule peptides in the complex was confirmed by SDS PAGE electrophoresis of small molecule peptides. The amount of polypeptide binding in the complex was determined by Bradfords method. The results showed a typical honeycomb submicron particle structure under electron microscopy. The amount of polypeptide bound in ISCOMs prepared was about 0.30 mg / ml. Conclusion The successful preparation of ISCOMs lays the foundation for further study on the immunological characteristics of immunostimulating complexes of small molecule peptides