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目的 观察一氧化氮 (NO)对哮喘大鼠基质金属蛋白酶 (MMP)及金属蛋白酶组织抑制物表达的影响 ,探讨其在哮喘气道结构重建中的作用。方法 30只雄性Wistar大鼠随机分为对照组、哮喘组和左旋精氨酸组 (L Arg组 ) ,每组 1 0只。肺组织作病理切片并HE染色 ,通过病理图像分析系统测定支气管基底膜周径 (Pbm)、总管壁面积 (WAt)、内壁面积 (WAi) ,平滑肌面积 (WAm)等形态学参数。用NO与一氧化氮合酶 (NOS)试剂盒测定肺组织中亚硝酸盐 /硝酸盐 (NO- 2 /NO- 3)水平与NOS活性。半定量逆转录聚合酶链反应技术 (RT PCR)分析肺组织中MMP 2与TIMP 1mRNA的表达。结果(1 )WAt/Pbm、WAi/Pbm及WAm/Pbm哮喘组 [分别为 (2 5 3± 2 1 ) μm2 / μm、(2 0 4± 2 3) μm2 / μm、(4 2±2 0 ) μm2 / μm]和L Arg组 [分别为 (35 1± 2 6) μm2 / μm、(2 5 3± 2 0 ) μm2 / μm、(8 7± 1 5) μm2 / μm]与对照组 [分别为 (2 0 8± 1 3) μm2 / μm、(1 5 3± 2 1 ) μm2 / μm、(3 1± 1 1 ) μm2 / μm]比较 ,差异有显著性(P <0 0 1 ) ;L Arg组与哮喘组比较差异亦有显著性 (P <0 0 5)。 (2 )肺组织中NO- 2 /NO- 3水平哮喘组[(7 2± 2 1 )nmol/mg]和L Arg组 [(1 1 8± 1 7)nmol/mg]与对照组 [(3 1± 1 2 )n
Objective To observe the effect of nitric oxide (NO) on the expression of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinases (TIMPs) in asthmatic rats and the role of nitric oxide (NO) in airway structure remodeling in asthmatic rats. Methods Thirty male Wistar rats were randomly divided into control group, asthma group and L Arg group (10 rats in each group). The pathological sections of lung tissue were made and stained with HE. Morphological parameters including bronchial basement membrane (PBm), total wall area (WAt), internal wall area (WAi) and smooth muscle area (WAm) were measured by pathological image analysis system. NO and nitric oxide synthase (NOS) kits were used to determine nitrite / nitrate (NO-2 / NO-3) levels and NOS activity in lung tissue. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze the expression of MMP 2 and TIMP-1 mRNA in lung tissue. RESULTS: (1) The WAt / Pbm, WAi / Pbm and WAm / Pbm asthma groups were respectively (2 53 ± 2 1) μm 2 / μm, (2 0 4 ± 2 3) μm 2 / μm, (4 2 ± 2 0 ) and L Arg group [(35 1 ± 2 6) μm 2 / μm, (2 5 3 ± 2 0) μm 2 / μm and (8 7 ± 1 5) μm 2 / μm] The difference was significant (P <0.01) when compared with the control group (20.8 ± 1 3) μm 2 / μm, (15 3 ± 2 1) μm 2 / μm and (3 1 ± 1 1) μm 2 / There was also significant difference between L Arg group and asthma group (P <0.05). (2) The levels of NO-2 / NO-3 in the asthma group were (72 ± 21) nmol / mg and [ 3 1 ± 1 2) n