论文部分内容阅读
目的观察光化学法诱导双侧海马梗死后大鼠的学习记忆功能及组织病理改变,为研究康复训练对学习记忆功能的影响建立动物模型。方法30只大鼠随机分为模型组和正常组,模型组用70mg/kg体重的虎红联合冷光源照射双侧海马,3d后观察其神经行为学及组织病理学改变,以评估模型的可行性。结果光镜下模型组双侧海马内有明确梗死灶,梗死面积恒定。与对照组相比,大鼠双侧海马梗死后神经行为学改变明显(P<0.01)。结论光化学法诱导双侧海马梗死后大鼠的学习记忆功能明显下降,有与海马梗死相符的组织病理改变,梗死模型稳定可靠。
Objective To observe the learning and memory function and histopathological changes of rats after bilateral hippocampal infarction induced by photochemical method and to establish an animal model for studying the effect of rehabilitation training on learning and memory function. Methods Thirty rats were randomly divided into model group and normal group. The bilateral hippocampus were irradiated with tiger red combined with cold light of 70 mg / kg body weight in model group, and the neurobehavioral and histopathological changes were observed after 3d to evaluate the feasibility of the model Sex. Results In the model group, there were definite infarcts in the bilateral hippocampus and the area of infarction was constant. Compared with the control group, neurobehavioral changes of bilateral hippocampal infarction in rats were significant (P <0.01). Conclusions After photochemical therapy, the learning and memory abilities of rats with bilateral hippocampal infarction were significantly decreased. The histopathological changes coincided with the hippocampal infarction. The infarction model was stable and reliable.