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应用免疫组织化学方法探讨抗呆Ⅰ号对小鼠前脑缺血再灌注损伤后海马星形胶质细胞表达GFAP动态变化的影响,其与缺血性神经元的联系。结果显示:前脑缺血再灌注1d只有少数星形胶质细胞表达GFAP,再灌注3d表达GFAP的阳性细胞数增加,再灌注7～10d表达GFAP的阳性细胞数达到高峰,同时,可见细胞反应性胶质化特征,前脑缺血再灌注15dGFAP阳性反应细胞数仍维持在较高水平。用药组GFAP的表达细胞数较模型组明显减少。而正常对照组和假手术组只有少数星形胶质细胞表达GFAP。且星形胶质细胞表达GFAP的强弱与神经细胞的存亡有关。说明前脑缺血再灌注后海马反应性星形胶质细胞表达。GFAP呈动态变化,抗呆Ⅰ号对缺血再灌注损伤星形胶质细胞和神经细胞有良好的保护作用。
Immunohistochemistry was used to investigate the effect of Kangdai I on the dynamic changes of GFAP expression in hippocampal astrocytes after forebrain ischemia-reperfusion in mice, and its relationship with ischemic neurons. The results showed that only a few astrocytes expressed GFAP on the first day after cerebral ischemia/reperfusion, and the number of GFAP-expressing cells increased after 3 days of reperfusion. The number of GFAP-expressing cells reached a peak at 7-10 days after reperfusion, and at the same time, the cellular response was seen. Glial characteristics, forebrain ischemia-reperfusion 15dGFAP positive reaction cells remained at a high level. The number of cells expressing GFAP in the drug group was significantly reduced compared with the model group. However, only a few astrocytes express normal GFAP in normal and sham groups. The strength of astrocytes expressing GFAP is related to the survival of nerve cells. This study demonstrated the expression of hippocampal reactive astrocytes after forebrain ischemia-reperfusion. GFAP showed dynamic changes, and Kangdai I had a good protective effect on astrocytes and neurons after ischemia-reperfusion injury.