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目的：利用反义寡核着酸阻遏血管平滑肌细胞（VSMC）c-myC，PCNA基因表达，为移植血管再狭窄的基因治疗提供理论依据．方法：人工合成正义、反义及错配反义c-myc，PCNA基因片断，并转人体外培养的VSMC中，运用逆转录聚合酶链式反应（RT-PCR）测定细胞中相应基因表达的情况．结果：反义寡核苦酸能有效抑制VSMC中c-myc，PC-NA基因的表达，而正义和错配反义寡核着酸没有此效应．结论：反义寡核着酸能阻遏VSMC中相应基因的表达．
OBJECTIVE: To use antisense oligonucleotides to inhibit the expression of c-myC and PCNA in vascular smooth muscle cells (VSMCs), and to provide a theoretical basis for the gene therapy of transplanted vascular restenosis. Methods: Sense, antisense and mismatched antisense c-myc and PCNA gene fragments were synthesized and transfected into VSMCs cultured in vitro. The expression of corresponding genes in the cells was detected by reverse transcription-polymerase chain reaction (RT-PCR) Happening. RESULTS: Antisense oligonucleotides inhibited the expression of c-myc and PC-NA genes in VSMCs, whereas sense and mismatched antisense oligodeoxynucleotides did not. CONCLUSION: Antisense oligonucleotides inhibit the expression of the corresponding genes in VSMCs.