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根据苏云金杆菌（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓ）杀虫晶体蛋白基因的结构特点，合成了ｃｒｙⅠ基因５′端特异引物和３′端共同引物，利用ＰＣＲ扩增，使不同基因引物产生不同长度的扩增产物，通过扩增产物片段的分子量大小来确定菌株所含杀虫基因类型．本研究对福建农业大学生物技术中心分离和保存的２０个菌株进行了ｃｒｙⅠＡ（ａ～ｃ）基因类型的ＰＣＲ鉴定．结果表明：４个菌株含有ｃｒｙⅠＡ（ａ）、ｃｒｙⅠＡ（ｂ）和ｃｒｙⅠＡ（ｃ）基因，２个菌株含有ｃｒｙⅠＡ（ａ）和ｃｒｙⅠＡ（ｃ）基因，其余菌株不含所鉴定的基因类型．
According to the structural characteristics of Bacilusthuringiensis insecticidal crystal protein gene, the 5 ’end cry1 gene specific primer and the 3’ end common primer were synthesized. By PCR amplification, different gene primers were used to generate amplification products of different length. Increase the molecular weight of fragments to determine the type of insecticidal strains contained in the gene. In this study, 20 strains isolated and preserved in Biotechnology Center of Fujian Agriculture University were identified by cry Ⅰ A (a ~ c) genotyping. The results showed that four strains contained cry Ⅰ A (a), cry Ⅰ A (b) and cry Ⅰ A (c) genes, two strains contained cry Ⅰ A (a) and cry Ⅰ A (c) genes, and the other strains did not contain the identified genotypes.