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目的研究脑胶质瘤组织不同部位O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)启动区甲基化状态及其表达的差异性。方法在54例脑胶质瘤组织的中心和周边部位分别获取标本,共获得92份标本,采用巢式甲基化特异性PCR(MSP)进行MGMT启动区甲基化状态的检测,同时采用免疫组织化学方法进行MGMT蛋白表达的检测。结果 38例胶质瘤中获得了肿瘤两个不同部位MG-MT启动区甲基化的状态,其中24例胶质瘤MGMT启动区甲基化的状态是一致的,占总数的63.2%(24/38)。在29例胶质瘤患者中获得了肿瘤两个不同部位MGMT蛋白表达的情况,其中10例胶质瘤MGMT蛋白表达是一致的,占总数的34.5%(10/29)。两总体一致性概率间差值的95%置信区间为(5.6%和51.8%),两者的差异有统计学意义。巢式MSP和免疫组化都获得检测结果的标本有77份,在61份MGMT启动区甲基化标本中,25份MGMT蛋白表达阴性,14份蛋白表达可疑阳性,18份蛋白表达阳性,4份蛋白表达强阳性。在16份MGMT启动区非甲基化标本中,2份MGMT蛋白表达阴性,2份蛋白表达可疑阳性,9份蛋白表达阳性,3份蛋白表达强阳性,MGMT启动区甲基化状态与蛋白表达呈负相关(r=-0.318,P=0.005)。结论脑胶质瘤MGMT蛋白表达在同一肿瘤的不同部位存在明显的异质性。虽然脑胶质瘤MGMT启动区甲基化的状态在同一肿瘤的不同部位存在一定的异质性,但是大多数脑胶质瘤MGMT启动区甲基化的状态在同一肿瘤的不同部位是一致的。脑胶质瘤MGMT启动区甲基化状态的一致性优于蛋白表达的一致性。脑胶质瘤MGMT启动区甲基化,MGMT蛋白表达较少,MGMT启动区非甲基化,MGMT蛋白表达较多。
Objective To investigate the methylation status and its expression of promoter region of O6-methylguanine-DNA methyltransferase (MGMT) in different parts of glioma tissues. Methods Totally 92 samples were obtained from 54 cases of glioma. The methylation status of MGMT promoter was detected by nested methylation-specific PCR (MSP) Histochemical methods for the detection of MGMT protein expression. Results The methylation status of MG-MT promoter in two different sites was obtained in 38 gliomas. The methylation status of MGMT promoter was consistent in 24 gliomas, accounting for 63.2% of the total / 38). The expression of MGMT protein in two gliomas was obtained in 29 patients with glioma. The expression of MGMT protein was consistent in 10 gliomas, accounting for 34.5% (10/29) of the total. The 95% confidence intervals between the two overall consistency probabilities were (5.6% and 51.8%), respectively, with significant differences between the two. 77 samples were detected by nested MSP and immunohistochemistry. Among the 61 MGMT promoter methylation samples, 25 MGMT protein was negative, 14 protein was positive, 18 protein was positive, 4 Strong protein expression of positive. Of the 16 MGMT promoter non-methylated samples, 2 MGMT protein negative, 2 protein suspicious positive, 9 protein positive, 3 protein strong positive, MGMT promoter methylation status and protein expression Was negatively correlated (r = -0.318, P = 0.005). Conclusion The expression of MGMT protein in glioma has obvious heterogeneity in different parts of the same tumor. Although the methylation status of MGMT priming region in glioma is heterogeneous in different parts of the same tumor, the methylation status of most glioma MGMT priming regions is consistent in different parts of the same tumor . The consistency of methylation status of glioma MGMT priming region is better than that of protein expression. MGMT promoter region methylation, MGMT protein expression less, MGMT promoter non-methylation, MGMT protein expression more.