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目的:对脑缺血大鼠采用尼莫地平与石杉碱甲进行药物干预治疗,观察治疗后不同药物组血管内皮生长因子(VEGF)、caspase-3表达水平与学习记忆改善程度。方法:采用免疫组化和Y迷宫检测脑缺血鼠学习记忆功能的变化及VEGF、caspase-3在缺血区的表达。结果:各治疗组VEGF、caspase-3在海马区、大脑皮质、基底节区表达均显著低于脑缺血对照组(A组)P<0.01,尤以海马、大脑皮质区较显著。其中石杉碱甲加尼莫地平干预组(B组)VEGF、caspase-3细胞阳性表达的降低和学习记忆功能的改善,明显好于A、C、D组,P<0.01。结论:石杉碱甲加尼莫地平治疗组与其他药物治疗组比,能有效改善VEGF、caspase-3表达水平和提高学习记忆的能力,为认知障碍早期的治疗提供依据。
Objective: To observe the effect of nimodipine and huperzine A on the expression of vascular endothelial growth factor (VEGF), caspase-3 and learning and memory in different medicine groups after treatment. Methods: The changes of learning and memory function in cerebral ischemia rats and the expression of VEGF and caspase-3 in ischemic area were detected by immunohistochemistry and Y-maze. Results: The expressions of VEGF and caspase-3 in hippocampus, cerebral cortex and basal ganglia were significantly lower than those in control group (P <0.01), especially in hippocampus and cerebral cortex. The decrease of positive expression of VEGF and caspase-3 cells in Huperzine A plus nimodipine intervention group (B group) and the improvement of learning and memory function were significantly better than those in A, C and D groups (P <0.01). Conclusion: Compared with other drugs, Huperzine A and Nimodipine can effectively improve the expression of VEGF and caspase-3 and improve the ability of learning and memory, so as to provide basis for early treatment of cognitive impairment.