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白介素21(IL-21)是最新发现的与白介素2,白介素14和白介素15结构和序列同源的细胞因子.对于T细胞、B细胞和NK细胞有提高免疫应答等多种生物学活性.所以白介素21特别是人源白介素是一个抗肿瘤治疗和其他免疫治疗的一个潜在活性蛋白.为进一步研究IL-21,从刺激过的人外周血单核细胞中成功克隆成熟人白介素21编码基因,经过大肠杆菌密码子偏好的生物信息学分析、突变密码子、转化、诱导和CM-52阳离子交换柱分离纯化,实现了重组人白介素21蛋白在pET22b系统中的无标签表达.并将重组蛋白应用于淋巴细胞增殖实验和直接抗肿瘤(HepG2,K562和MDA)活性检测体外活性检测.结果表明,淋巴细胞增殖与重组人白介素21浓度成正相关,而r hIL-21对人肿瘤细胞无直接抗肿瘤活性.“,”Interleukin 21 (IL-21) is a newly discovered cytokine with structural and sequence homology to IL-2, IL-4 and IL-15. It is reported with functions of elevating anti-tumour immune responses as well as sev-eral special biological activities for T cell, B cell and natural killer cell, thus IL-21 especially human IL-21 (Hil-21) is a potential protein for anti-tumour therapy and other immunotherapy. To further research into Hil-21, the code gene of matured human IL-21 (Hil-21) from stimulated peripheral blood mononuclear cells (PBMC) was cloned and mutated to realize the high expression without tag protein in pET22b expression vector after bioinformatics analysis of E. Coli eodon preference. Recombinant Hil-21 ( R Hil-21 ) was successfully gained after isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducing, periphplasm diosmosis and CM-52 chro-matography, r Hil-21 protein was then applied to lymphocyte proliferation assay in vitro. Lymphocytes prolif-eration correlated with r Hil-21 concentration.