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（１）目的：多数重症肌无力（ＭＧ）患者可见作为烟碱样乙酰胆碱受体（ｎＡＣｈＲ）抗体（Ａｂ）升高基础的抗原特异性辅助性Ｔ细胞（Ｔｈ）增多，本研究目的在于探讨此类细胞的可能作用。（２）方法：设重症肌无力、其它神经科患者和正常对照三组。用体外测试的免疫酶点法，即以电鳗的ＡＣｈＲα－，β－，γ－，和δ－亚单位作为特异性抗原以及ＰＰＤ和与多发性硬化相关的髓鞘碱性蛋白作为非特异性抗原，于抗原刺激后计数分泌γ干扰素（ＩＦＮ－γ）的Ｔｈ１细胞数。测定周围血中针对这些抗原的Ｔ细胞亚群计数。（３）结果：ＭＧ患者识别ＡＣｈＲ四种亚单位的Ｔ细胞数均增高，依次为：１／２５０００，１／５９０００，１／８３０００和１／２５０００个细胞。６５％ＭＧ患者的Ｔ细胞主要识别α－亚单位，有些则主要识别γ－和δ－亚单位。少数ＭＧ患者测不到对任何亚单位起反应的Ｔ细胞。（４）结论：ＭＧ患者中，ＡＣｈＲ的四种亚单位均为通过分泌ＩＦＮγ的Ｔｈ１样细胞自身免疫攻击的靶。
(1) Objective: In the majority of patients with myasthenia gravis (MG), an increase in antigen-specific helper T cells (Th), which is the basis for elevated nicotinic acetylcholine receptor (Ab), is seen in this study Possible role of cells. (2) method: set myasthenia gravis, other neurological patients and normal control three groups. In vitro immunoenzymatic assays, ie, the use of AChRα-, β-, γ-, and δ-subunits of electric eels as specific antigens and PPD and myelin basic proteins associated with multiple sclerosis as nonspecific antigens The number of Th1 cells secreting gamma interferon (IFN-γ) was counted after antigen stimulation. T cell subsets counts against these antigens were determined in peripheral blood. (3) Results: The number of T cells recognizing AChR four subunits in MG patients were all higher: 1/25000, 1/59000, 1/83000 and 1/25000 cells. T cells in 65% of MG patients mainly recognize the α-subunit, while others mainly recognize γ- and δ-subunits. A small number of MG patients can not detect any subunit-responsive T cells. (4) CONCLUSIONS: Among the MG patients, all four subunits of AChR are targets of autoimmune attack by Th1-like cells secreting IFNγ.