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采用基于核磁共振波谱技术的代谢组学技术,探索菲律宾蛤仔在受到鳗弧菌和灿烂弧菌感染的代谢物变化特征,构建菲律宾蛤仔对弧菌感染后的代谢网络调控图谱,并比较两种弧菌毒性效应的差异。试验结果表明,3种代谢物葡萄糖、谷氨酸、苏氨酸在两种弧菌感染时均发生了变化,表征鳗弧菌感染的代谢物为牛磺酸、精氨酸、酪氨酸、苯丙氨酸、赖氨酸、亮氨酸、异亮氨酸、缬氨酸;而表征灿烂弧菌污染的代谢物为甜菜碱、二甲基甘氨酸、胆碱、谷氨酸、亚牛磺酸。
Metabonomics technology based on nuclear magnetic resonance spectroscopy was used to explore the characteristics of metabolites of Ruditapes philippinarum infected by Vibrio anguillarum and Vibrio sp., And the metabolic network regulation map of Ruditapes philippinarum infected by Vibrio spp. Differences in Toxic Effects of Vibrio species. The results showed that the three metabolites glucose, glutamic acid and threonine changed during the two Vibrio infections, and the metabolites of Vibrio anguillarum infection were taurine, arginine, tyrosine, Phenylalanine, lysine, leucine, isoleucine, and valine. The metabolites that characterize the sp. Poliovirus were betaine, dimethylglycine, choline, glutamic acid, acid.