目的研究临床标本分离的鲍曼不动杆菌的耐药性及其变化,为临床合理使用抗菌药物提供依据。方法用细菌分离鉴定技术和药敏试验方法,对某医院临床科室送检病人标本进行细菌学检验与分析。结果该医院连续3年共检测临床送检病原学标本32 166份,检出病原菌12 805株;其中检出鲍曼不动杆菌1 574株,检出率为4.9%,占病原菌总数的12.3%。鲍曼不动杆菌感染主要分布在重症医学科、呼吸内科、急诊科、神经外科。临床分离的鲍曼不动杆菌对临床常用抗菌药物耐药率均超过60%,对亚胺培南的耐药率也超过60%。结论该医院临床送检标本中鲍曼不动杆菌分离率较高,呈现严重耐药,应加强耐药菌监测和抗菌药物使用管理。 Objective To study the drug resistance and the changes of Acinetobacter baumannii isolated from clinical specimens and provide evidence for the rational use of antibacterials in clinic. Methods Bacterial isolation and identification technology and drug susceptibility testing methods were used to carry out bacteriological examination and analysis on the specimens of patients in a hospital clinical department. Results A total of 32 166 clinical pathogenic specimens were detected in the hospital for 3 consecutive years and 12 805 pathogens were detected. Of these, 1 574 Acinetobacter baumannii strains were detected, the detection rate was 4.9%, accounting for 12.3% of the total number of pathogenic bacteria. . Acinetobacter baumannii infection mainly in the Department of Critical Care Medicine, respiratory medicine, emergency department, neurosurgery. Acinetobacter baumannii clinical isolates of commonly used antimicrobial resistance rate of more than 60%, resistance rate to imipenem more than 60%. Conclusion The clinical isolates of Acinetobacter baumannii have a high isolation rate and serious resistance. The monitoring of drug-resistant bacteria and the administration of antibacterial drugs should be strengthened.