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目的:研究乳腺癌患者外周血hMAMmRNA,探讨其作为外周血微转移指标对临床的应用价值。方法:采用RT蛳PCR技术检测97例乳腺癌病例外周血hMAMmRNA,观察其个体临床病理参数,并与血清指标CEA、CA153作相关分析。结果:在肿瘤原发灶>2 cm、≤2 cm两组间,外周血hMAMmRNA阳性表达差异有统计学意义(χ2=8.13,P<0.05);淋巴结有无转移两组间差异有统计学意义(P<0.05);有无远处转移两组间差异有统计学意义(P<0.01);对于远处转移的乳腺癌患者,外周血hMAMmRNA、CA153、CEA的检测结果敏感性无统计学意义(P>0.05),但hMAMmRNA与后两者任一联合检测敏感性较单一指标明显提高(P<0.05),并且hMAMmRNA特异性高。结论:外周血hMAMmRNA阳性率可以反映乳腺癌的病期。联合检测外周血hMAMmRNA、CEA、CA153可以提高复发转移指标的敏感性与特异性。
Objective: To study the hMAM mRNA in peripheral blood of patients with breast cancer and explore its clinical value as a marker of peripheral blood micrometastasis. Methods: RT-PCR was used to detect hMAM mRNA in peripheral blood of 97 cases of breast cancer. The clinicopathological parameters were observed and correlated with serum CEA and CA153. Results: The positive expression of hMAM mRNA in peripheral blood was significantly different between the two groups (χ2 = 8.13, P <0.05). There was significant difference between the two groups (P <0.05). There was significant difference between the two groups with or without distant metastasis (P <0.01). The sensitivity of hMAMmRNA, CA153 and CEA in peripheral blood of patients with distant metastasis was not statistically significant (P> 0.05). However, the combined sensitivity of hMAM mRNA and the latter two indexes was significantly higher than that of single index (P <0.05), and the specificity of hMAM mRNA was high. Conclusion: The positive rate of hMAM mRNA in peripheral blood can reflect the stage of breast cancer. Combined detection of peripheral blood hMAM mRNA, CEA, CA153 can improve the sensitivity and specificity of recurrence and metastasis.