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蓝藻生物量检测技术发展是应对目前频繁发生的水华事件的重要环节。藻蓝蛋白作为蓝藻的特异性蛋白,在一定程度上比叶绿素更能准确反应自然水体中的蓝藻生物量,因而成为蓝藻生物量检测技术的重要指标。本文利用三维荧光光谱技术,以不同光照、不同生长期的铜绿微囊藻、鱼腥藻活体为研究对象,比较了单点法和包络法两种光谱解析方法的可靠性,探究了不同生境条件下的藻蓝蛋白活体荧光光谱特性。结果表明:(1)荧光光谱强度随生长期延长而增大;(2)采用包络法解析藻蓝蛋白特征荧光光谱的方法比单点法更为可靠;(3)在不同生境条件下,铜绿微囊藻藻蓝蛋白活体荧光激发波长基本保持614nm、发射波长基本保持654nm不变,鱼腥藻藻蓝蛋白活体荧光激发波长随生长期在610和620nm之间波动减小,发射波长随生长期在650和660nm之间波动增大。这种波动与藻种样品颗粒度大小和光谱扫描模式有关。该研究结果为发展蓝藻生物量活体荧光监测技术发展提供了实验基础。
The development of cyanobacteria biomass detection technology is an important part of coping with the current frequent bloom events. As a specific protein of cyanobacteria, phycocyanin can reflect the biomass of cyanobacteria in natural water more accurately than chlorophyll, and thus become an important indicator of cyanobacteria biomass detection technology. In this paper, three-dimensional fluorescence spectroscopy was used to study the reliability of two kinds of spectroscopic methods, one-point method and envelope method, in different light and different growth stages of Microcystis aeruginosa and Anabaena. Fluorescence Spectra of Phycocyanin in Vivo. The results showed that: (1) the fluorescence intensity increased with the prolongation of growth period; (2) the method of envelope method was more reliable than the single-point method in analyzing the characteristic fluorescence spectra of phycocyanin; (3) Under different habitat conditions, The fluorescence excitation wavelength of Microcystis aeruginosa phycocyanin remained essentially at 614 nm with the emission wavelength kept at 654 nm. Fluorescence excitation wavelength of A. fumigatus fluorescence decreased with the growth period between 610 and 620 nm. The emission wavelength increased with the growth Period of fluctuations between 650 and 660nm increased. This fluctuation is related to the particle size of the algae sample and the spectral scanning pattern. The results provide experimental basis for the development of cyanobacteria biomass fluorescence monitoring technology.