甘蔗蔗糖转运蛋白ShSUT4基因克隆及表达分析

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采用RACE技术从甘蔗体内克隆出一个新型蔗糖转运蛋白基因,命名为ShSUT4(GenBank登录号为GQ485583.1),预测其编码501个氨基酸,存在GPH(蔗糖/H+共转运体)超家族保守结构域,具有12跨膜结构。初步研究结果表明,ShSUT4在甘蔗根茎叶中均有表达;且在9个不同甘蔗品种成熟茎中表达量存在差异,在其中8个品种中ShSUT4表达量的高低与所测茎节中锤度高低呈正相关性,推测ShSUT4可能参与调控甘蔗体内蔗糖的积累。 A novel sucrose transporter gene named ShSUT4 (GenBank accession number: GQ485583.1) was cloned from sugarcane by RACE technology and predicted to encode 501 amino acids. There was a conserved domain of GPH (sucrose / H + co-transporter) superfamily , With 12 transmembrane structures. Preliminary results showed that ShSUT4 was expressed in roots, stems and leaves of sugarcane, and there were differences in the expression levels of ShSUT4 among the nine varieties of sugarcane cultivars. The expression level of ShSUT4 in the eight cultivars was similar to that in stems It is speculated that ShSUT4 may be involved in the regulation of sucrose accumulation in sugarcane.
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