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水稻叶片的最大光合速率出现在上午１０：００时，Ｒｕｂｉｓｃｏ初始活力也在此时达到最大，然后逐渐降低，下午１４：００时略上升后又下降．Ｒｕｂｉｓｃｏ初始活力与光合速率之间极显著相关，相关系数为 ０．９４７４．运用相关性分析、回归分析、通径分析，对晴（有时有云）天气水稻叶片的光合速度、光合关键酶及有关因素的日变化进行综合评估，结果发现中午光合下降主要来自气孔限制，同时Ｒｕｂｉｓｃｏ活性也下降；Ｒｕｂｉｓｃｏ活性是影响光合日变化的又一重要生化因子．体内Ｒｕｂｉｓｃｏ活性受Ｒｕｂｉｓｃｏ活化酶的调节．
The maximum photosynthetic rate of rice leaves at 10:00 in the morning, Rubisco initial activity also reached the maximum at this time, and then gradually decreased at 14:00 slightly increased and then decreased. Rubisco initial vigor and photosynthetic rate were significantly correlated, with a correlation coefficient of 0.9474. The correlation analysis, regression analysis and path analysis were used to evaluate the diurnal variation of photosynthetic rate, photosynthetic key enzymes and related factors in sunny (sometimes cloudy) weather rice leaves. The results showed that the decrease of photosynthetic rate was mainly caused by stomatal limitation, meanwhile, Rubisco activity also decreased; Rubisco activity is another important biochemical factor affecting photosynthetic diurnal changes. Rubisco activity in vivo is regulated by Rubisco activase.