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目的:探讨人附睾蛋白4(human epididymis protein 4,HE4)在子宫内膜癌患者血清及组织中的表达及临床意义。方法:ELISA法检测子宫内膜癌组40例、子宫良性疾病组42例及正常对照组40例血清HE4水平,免疫组织化学检测上述子宫内膜癌、配对的癌旁组织及子宫良性疾病组组织中HE4的表达。结果:子宫内膜癌组血清HE4水平较子宫良性疾病组及正常对照组明显升高(P<0.01),子宫内膜癌组术后血清HE4水平较术前明显下降(P<0.01),子宫内膜癌组织HE4表达分别高于配对的癌旁组织及子宫良性疾病内膜组织(P<0.01),子宫内膜癌患者血清及组织HE4表达阳性率在不同的临床分期、肿瘤的分化程度、病灶范围、肌层浸润深度及淋巴结转移与否比较差异有统计学意义(P<0.05)。结论:子宫内膜癌患者血清及组织中HE4表达水平升高,而且其表达水平与肿瘤临床分期、分化程度、病灶大小、浸润程度及有无淋巴结转移有关,HE4可能作为子宫内膜癌辅助诊断、病情评估及预后判断的生化指标之一。
Objective: To investigate the expression of human epididymis protein 4 (HE4) in the serum and tissues of patients with endometrial carcinoma and its clinical significance. Methods: The levels of serum HE4 in 40 cases of endometrial cancer, 42 cases of benign uterine disease and 40 cases of normal control group were detected by ELISA. The expression of endometrial carcinoma, matched paracancerous tissues and benign uterine disease tissues were detected by immunohistochemistry In HE4 expression. Results: The serum level of HE4 in endometrial carcinoma group was significantly higher than that in benign disease group and normal control group (P <0.01), while the level of HE4 in endometrial carcinoma group was significantly lower than that before operation (P <0.01) The expression of HE4 in endometrial carcinoma tissues was higher than that in paired paracancerous tissues and benign endometrial tissues (P <0.01). The positive rates of HE4 in serum and tissues of endometrial carcinoma were significantly different in different clinical stages, tumor differentiation, The extent of the lesion, the depth of myometrial invasion and lymph node metastasis or not were significantly different (P <0.05). Conclusion: The expression of HE4 in serum and tissues of patients with endometrial carcinoma is elevated, and its expression level is related to clinical stage, differentiation degree, size of lesions, degree of infiltration and lymph node metastasis. HE4 may be used as an auxiliary diagnosis of endometrial cancer , Disease assessment and prognosis of biochemical indicators.