【摘 要】
：
为了建立鸭先天免疫受体核苷酸寡域1（NOD1）的荧光定量RT-PCR检测方法,依据GenBank中鸡NOD1序列设计特异性引物,克隆获得鸭NOD1序列,设计鸭NOD1、凋亡相关碱性蛋白（ARBP）荧光定量R
【机 构】
：
安徽农业大学动物科技学院,中国农业科学院上海兽医研究所
论文部分内容阅读
为了建立鸭先天免疫受体核苷酸寡域1（NOD1）的荧光定量RT-PCR检测方法,依据GenBank中鸡NOD1序列设计特异性引物,克隆获得鸭NOD1序列,设计鸭NOD1、凋亡相关碱性蛋白（ARBP）荧光定量RT-PCR特异性引物,以鸭脾脏mRNA反转录后的cDNA为模板,通过构建质粒阳性标准品,建立检测鸭先天免疫受体NOD1转录水平的荧光定量RT-PCR方法.结果显示,建立的荧光定量RT-PCR方法对NOD1 mRNA的最低检测量为102拷贝,在1012～102拷贝范围内线性关系很好,R2≥0.999.该方
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