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本研究应用聚合酶链反应（ＰＣＲ）产物的凝胶电泳及Ｓｏｕｔｈｅｒｎ－ｂｌｏｔｔｉｎｇ，检测湖南４３份牛尿标本中的钩端螺旋体ＤＮＡ，前法阳性率为９．３％，后法为１４％，表明牛群具有较高的带菌率，是钩端螺旋体的主要宿主，是钩端螺旋体病的重要传染源。
In this study, PCR-based gel electrophoresis and Southern-blotting were used to detect Leptospira DNA in 43 specimens of cow urine from Hunan Province. The positive rate of the former method was 9.3% and the latter was 14% The results showed that the herd had the highest bacteriocidal rate and was the main host of Leptospira and was the main source of infection for Leptospirosis.