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应用液体培养法培养小鼠骨髓细胞获得了比较稳定且有一定数量血小板生成的培养体系。培养3、5、7、9d时体系中的血小板数均高于接种时。在培养7d时可见巨核细胞生成血小板的现象,~(35)S掺入也证实体外有血小板生成。这些体外生成的血小板形态功能基本正常,其直径为1—5μm,新生成的血小板体积较大。无论体积大小,其活动性均稍强于正常。这些体外生成的血小板具有正常粘附功能,2×10~(-4)mol/L ADP可诱导出单波聚集。体系中血小板及巨核细胞生成量稳定且与接种细胞数呈正相关,提示可将其应用于巨核系生成调控的研究。进一步增加并稳定血小板生成量可使此体系更有效地应用于血小板形态、功能及生成调控的研究。
Cultured mouse bone marrow cells using liquid culture method to obtain a more stable and a certain number of platelet-producing culture system. The number of platelets in the system at 3, 5, 7 and 9 d after culture was higher than that at the time of inoculation. The phenomenon of platelet production by megakaryocytes was observed at 7 days of culture. The incorporation of ~ (35) S also confirmed platelet production in vitro. These in vitro generated platelet morphology is basically normal, the diameter of 1-5μm, the new generation of larger platelets. No matter the size, its activity is slightly stronger than normal. These in vitro generated platelets have normal adhesion function, 2 × 10 -4 mol / L ADP can induce a single wave aggregation. The platelet and megakaryocyte production in the system was stable and positive correlation with the number of inoculated cells, suggesting that it can be applied to the regulation of megakaryocytopoiesis. Further increase and stabilize platelet production can make this system more effective in the study of platelet morphology, function and gene regulation.