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目的:研究COX-2抑制剂NS-398对Tca8113细胞系表达血管内皮生长因子(VEGF)的影响。方法:应用逆转录聚合酶链反应(RT-PCR)及Westernblot方法,在环氧合酶-2抑制剂NS-398作用的基础上,观察NS398对Tca8113细胞系表达VEGF的影响。结果:NS-398可下调Tca8113细胞内VEGF的表达,且表现出一定的时间依赖性关系。结论:NS-398可能在COX-2参与口腔鳞癌新生血管生成的过程中起重要作用。
Objective: To investigate the effect of COX-2 inhibitor NS-398 on the expression of vascular endothelial growth factor (VEGF) in Tca8113 cell line. Methods: The effect of NS398 on the expression of VEGF in Tca8113 cell line was observed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting based on the role of cyclooxygenase-2 inhibitor NS-398. Results: NS-398 down-regulated the expression of VEGF in Tca8113 cells and showed a time-dependent relationship. Conclusion: NS-398 may play an important role in the process of COX-2 involvement in angiogenesis in oral squamous cell carcinoma.