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目的：研究一种对酸热稳定的多肽（Ａｃｉｄ／ｈｅａｔ Ｓｔａｂｌｅ Ｐｏｌｙｐｅｐｔｉｄｅｓ，ＡＨＳＰ）制剂对伤口组织细胞增殖反应的作用及机理。方法：利用从猪血清中分离出的一组对酸热稳定的多肽（ＡＨＳＰ）以及从国外购得的牛血清除大分子蛋白后的多肽成分（Ｓｏｌｃｏｓｅｒｙｌ），观察两种多肽制剂在培养的伤口组织修复细胞及人表皮细胞增殖和细胞总蛋白合成能力中的作用。结果：当Ｓｏｌｃｏｓｅｒｙｌ和ＡＨＳＰ的浓度低于７％时，其对伤口修复细胞和人离体培养的表皮细胞的增殖和蛋白合成能力随浓度增加而增加，浓度为５％时，两者对细胞活力的促进作用明显强于相应浓度的小牛血清对照组；而与 １０％的小牛血清对照组相比无明显差异。 Ｓｏｌｃｏｓｅｒｙｌ和 ＡＦＳＰ的浓度为 ５％～ ７％时，曲线变化较为平缓，当浓度上升为 １０— １２％时， Ｓｏｌｃｏｓｅｒｙｌ和 ＡＨＳＰ组与相应浓度的小牛血清对照组相比有明显的抑制作用。结论：在伤口愈合过程中ＡＰＳＰ可能是通过提高细胞的酶活性，促进细胞的蛋白合成，进而刺激伤口组织细胞增生并促进伤口愈合的。
OBJECTIVE: To study the effect and mechanism of acid / heat Stable Polypeptides (AHSP) on the proliferation of wound tissue. Methods: Using a set of heat-stable peptides (AHSP) isolated from porcine serum and polypeptide fractions (Solcoseryl) after removal of macromolecules from bovine serum purchased from foreign countries, the effects of two polypeptide preparations on cultured wounds Tissue repair cells and human epidermal cell proliferation and total cellular protein synthesis capacity. Results: When the concentration of Solcoseryl and AHSP was less than 7%, the proliferation and protein synthesis ability of wound healing cells and human epidermal cells in vitro increased with increasing concentration. When the concentration of Solcoseryl and AHSP was 5% Was significantly stronger than the corresponding concentration of bovine serum control group; and 10% of the bovine serum control group no significant difference. When the concentration of Solcoseryl and AFSP was 5% ~ 7%, the curve changed more moderately. When the concentration increased to 10-12%, Solcoseryl and AHSP groups had obvious inhibition compared with the corresponding concentration of bovine serum control group. Conclusion: In the process of wound healing, APSP may promote cell proliferation and promote wound healing by increasing cellular enzymatic activity, promoting cellular protein synthesis.