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目的探讨TNF-α和TGF-β1在慢性非细菌性前列腺炎患者前列腺液中的表达及临床意义。方法采用双抗体夹心法对20例炎症型慢性非细菌性前列腺炎即炎症型慢性骨盆疼痛综合征(Ⅲa型),20例非炎症型慢性非细菌性前列腺炎即非炎症型慢性骨盆疼痛综合征(Ⅲb型),10例健康对照者前列腺液(EPS)中TNF-α、TGF-β1含量进行测定。采用美国国立卫生院慢性前列腺炎症状评分(NIH-CPSI)进行相关性研究。结果①TNF-α在Ⅲa型患者EPS中表达水平[(152.44±83.06)pg/ml]明显高于Ⅲb型组[(93.15±57.26)pg/ml]和健康对照组[(78.99±53.51)pg/ml],P<0.05。但在Ⅲb型和健康对照组之间差异无统计学意义,P>0.05。②TGF-β1在Ⅲa型组[(8477.50±4612.45)pg/ml]和Ⅲb型组[(7946.50±5044.06)pg/ml]表达水平均明显高于健康对照组[(2462.50±985.31)pg/ml],P<0.01。但在Ⅲa和Ⅲb型之间差异无统计学意义,P>0.05。③前列腺液中TNF-α、TGF-β1的水平与慢性前列腺炎症状评分无相关性(r=0.23,P>0.05;r=0.31,P>0.05)。结论前列腺液中细胞因子(TNF-α、TGF-β1)在慢性前列腺炎的病理学改变中起重要作用,可作为慢性前列腺炎的诊断依据之一。
Objective To investigate the expression and clinical significance of TNF-α and TGF-β1 in prostatic fluid of patients with chronic nonbacterial prostatitis. Methods Twenty patients with inflammatory non-bacterial chronic prostatitis, chronic inflammation of chronic pelvic pain syndrome (Ⅲa), 20 non-inflammatory chronic non-bacterial prostatitis (non-inflammatory chronic pelvic pain syndrome) (Type Ⅲb), and the content of TNF-α and TGF-β1 in 10 healthy subjects with prostatic fluid (EPS). The National Institutes of Health chronic prostatitis symptom score (NIH-CPSI) for correlation studies. Results ① The expression level of TNF-α in EPS of type Ⅲa patients [(152.44 ± 83.06) pg / ml] was significantly higher than that in type Ⅲb patients (93.15 ± 57.26 pg / ml) and healthy controls [(78.99 ± 53.51) pg / ml], P <0.05. However, there was no significant difference between Ⅲb and healthy controls (P> 0.05). ② The expression of TGF-β1 in group Ⅲa [(8477.50 ± 4612.45) pg / ml] and group Ⅲb [(7946.50 ± 5044.06) pg / ml] were significantly higher than those in healthy control [(2462.50 ± 985.31) pg / , P <0.01. However, there was no significant difference between Ⅲa and Ⅲb types (P> 0.05). ③ There was no correlation between the levels of TNF-α and TGF-β1 in prostatic fluid and symptom scores of chronic prostatitis (r = 0.23, P> 0.05; r = 0.31, P> 0.05). Conclusions Cytokines (TNF-α, TGF-β1) in prostatic fluid play an important role in the pathological changes of chronic prostatitis and may be used as a diagnostic basis for chronic prostatitis.