论文部分内容阅读
目的了解帕金森病(PD)模型大鼠在清醒静止状态下基底节-皮层环路的异常振荡性电活动。方法用6-羟基多巴胺(6-OHDA)脑内两点注射法建立PD大鼠模型,并经阿扑吗啡注射诱发旋转对模型进行评价。通过多导宏电极在体电生理记录技术结合视频录像,对可自由活动的正常大鼠和6-OHDA大鼠PD模型进行苍白球场电位和皮层脑电的多部位同时记录。功率谱分析和相干分析用于揭示各记录位点信号的频率成分以及不同记录位点神经元集群之间的功能连接和同步化。结果清醒静止状态下,苍白球场电位中存在特征明显的μ节律活动。与正常大鼠相比,PD模型大鼠苍白球场电位μ节律的持续时间明显延长;主运动皮层出现与苍白球同步的μ节律活动,特别是注射侧主运动皮层的μ节律被显著增强。结论μ节律是大鼠清醒静止状态下苍白球本身具有的一种生理性节律活动。6-OHDA对中脑多巴胺能神经元的损害可造成苍白球μ节律持续时间延长,还可改变基底节-皮层环路的功能连接,造成主运动皮层与苍白球之间异常同步的μ节律活动。至于此异常μ节律活动与PD静止性震颤的关系,尚需经进一步实验加以研究。
Objective To investigate the abnormal oscillatory electrical activity of basal ganglia-cortex loops in Parkinson’s disease (PD) rats under consciously awake conditions. Methods PD rat model was established by injecting 6-hydroxydopamine (6-OHDA) intracerebrally into two points and the model was evaluated by apomorphine injection induced rotation. Through the multi-transduction macroscopical electrophysiological recording technology combined with video recording, the plots of pallidal field potential and cortical EEG were recorded at the same time in the free-flowing PD model of normal rats and 6-OHDA rats. Power spectrum analysis and coherence analysis were used to reveal the frequency components of the signal at each recording site and functional connectivity and synchronization between the neuronal clusters at different recording sites. Results awake at rest, the pale field potential in the presence of significant μ rhythmic activity. Compared with normal rats, the duration of μ rhythm of globus pallidus in PD model rats was prolonged significantly. The μ rhythmic activity synchronized with globus pallidus appeared in the main motor cortex, especially the μ rhythm of the main motor cortex in injection side was significantly increased. Conclusion μ rhythm is a physiological rhythmic activity of the globus pallidus in rats at awake state. Impairment of midbrain dopaminergic neurons by 6-OHDA results in prolonged μ-rhythmic pallidolism and changes in functional connectivity of the basal ganglia-cortical circuits, resulting in abnormally synchronized μ-rhythmic activity between the main motor cortex and globus pallidus . As for the relationship between abnormal rhythmic activity and resting tremor of PD, we need further study.