作者用Nagai等的方法从卡介苗(BCG)的培养滤液中提取核糖体和核糖体亚单位蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析发现,在70S核糖体和30S亚单位中有一条65KDa蛋白带,为热休克蛋白(HSP)和类S1蛋白。这两种蛋白的分子量均为65KDa,与大肠杆菌的S1蛋白以及耻垢分枝杆菌的类S1蛋白的分子量相似。已知大肠杆菌S1蛋白能使信使RNA(mRNA)准确地与核糖体结合,耻垢分枝杆菌类S1蛋白与多聚尿苷 The authors extracted ribosomal and ribosomal subunit proteins from the culture filtrate of Bacillus Calmette-Guerin (BCG) using the method of Nagai et al. Sodium dodecyl sulfate - polyacrylamide gel electrophoresis analysis found that in the 70S ribosome and 30S subunits there is a 65KDa protein band, heat shock protein (HSP) and S1 protein. Both of these proteins have a molecular weight of 65 kDa, similar to the S1 protein of Escherichia coli and the S1 protein of M. smegmatis. It is known that Escherichia coli S1 protein enables messenger RNA (mRNA) to accurately bind ribosomes, and M. smegmatis S1 protein and polyuridine