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为研究高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)Hu N4株诱导断奶仔猪胸腺细胞凋亡的分子机制,本实验通过免疫荧光技术、流式细胞术、荧光定量PCR和western blot等方法检测HP-PRRSV感染仔猪胸腺内细胞凋亡相关因子Caspase-8、Caspase-9和Caspase-3表达量的变化。结果显示:HP-PRRSV感染仔猪后,胸腺内表达凋亡相关蛋白Caspase-8、Caspase-9和Caspase-3的细胞比例显著升高,并且凋亡相关蛋白Caspase-8、Caspase-9和Caspase-3表达量也明显升高。以上结果表明:HP-PRRSV感染仔猪引起胸腺细胞凋亡是通过Caspase依赖性通路介导。本研究为进一步了解HP-PRRSV感染机制提供了实验依据。
In order to study the molecular mechanism of HP-PRRSV Hu N4 induced apoptosis in thymocytes of weanling piglets, we used immunofluorescence, flow cytometry, real-time PCR and western blot And other methods to detect the changes of apoptosis-related factors Caspase-8, Caspase-9 and Caspase-3 in thymus of HP-PRRSV infected piglets. The results showed that the proportion of apoptosis-related proteins Caspase-8, Caspase-9 and Caspase-3 in thymus of HP-PRRSV infected piglets was significantly increased, and the apoptosis related proteins Caspase-8, Caspase-9 and Caspase- 3 expression was also significantly increased. The above results indicate that apoptosis of thymocytes induced by HP-PRRSV in piglets is mediated by Caspase-dependent pathways. This study provides experimental evidence for understanding the mechanism of HP-PRRSV infection.