论文部分内容阅读
研究了用HPLC 测定兔眼玻璃体和房水中维拉帕米含量的方法。用丙咪嗪为内标, 采用Spherical C18色谱柱分离, 以甲醇- 0 .04 mol/L 醋酸铵- 乙腈- 二乙胺( 体积比1 ∶1 ∶0 .5 ∶0 .02) 为流动相, 检测波长为278nm 。样品用正己烷- 异丁醇提取后进样, 分析速度快。 样品及内标的保留时间分别为4 .63 min 及9 .23 min ; 线性范围为2 .5 ~100 mg/L, 相关系数r 为0 .999 7 。方法的回收率为100 .3 % ( n = 5) ,RSD 为3 .63% , 日内RSD 为0 .5 % ~3 .61 % , 日间RSD 为3 .75 % ~4 .80 % , 最低检测质量浓度为0.25 mg/ L。 应用该法测定了创伤家兔玻璃体和房水中维拉帕米浓度的变化。
A method for the determination of verapamil in the vitreous and aqueous humor of rabbit eyes by HPLC was studied. With imipramine as internal standard, using Spherical C18 column separation, methanol - 0. 04 mol / L ammonium acetate - acetonitrile - diethylamine (volume ratio 1: 1: 0. 5: 0. 02) as the mobile phase, the detection wavelength of 278nm. Samples were extracted with n-hexane - isobutanol injection, the analysis speed. Sample and internal standard retention time was 4. 63 min and 9. 23 min; linearity is 2. 5 ~ 100 mg / L, the correlation coefficient r is 0. 999 7. The recovery rate of the method is 100. 3% (n = 5) with RSD of 3. 63%, day RSD is 0. 5% ~ 3. 61% and daytime RSD is 3. 75% ~ 4. 80%, the lowest detection mass concentration of 0.25 mg / L. The method was used to determine the changes of verapamil concentration in trauma rabbits vitreous and aqueous humor.