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目的研究决明子提取物对溴酸钾(KBrO3)/伤寒沙门氏菌脂多糖(LPS)诱发小鼠晶状体氧化应激状态的改善作用。方法小鼠尾静脉注射2 mg.kg-1LPS,同时腹腔注射200 mg.kg-1KBrO3制备小鼠晶状体氧化应激模型。50,150和450 mg.kg-1决明子提取物在LPS/KBrO3给药同时及3 h前分别灌胃2次,对照组灌胃同体积水溶液。LPS/KBrO3给药6 h后,乙醚麻醉条件下采取小鼠晶状体测定抗氧化能力指标。结果单独腹腔注射200 mg.kg-1KBrO3或尾静脉注射2 mg.kg-1LPS 6 h后,小鼠晶状体组织内谷胱甘肽(GSH)水平均有所降低,而当KBrO3并用LPS时则显著诱发小鼠晶状体氧化应激状态。50,150及450 mg.kg-1决明子提取物灌胃给药能够有效的改善因KBrO3/LPS诱导小鼠晶状体组织内GSH含量的减少、提高抗氧化能力指数(ORAC)和降低丙二醛(MDA)水平,并呈一定的量效关系。结论决明子提取物可以改善小鼠晶状体内的过氧化状态,其作用机制可能是通过清除自由基和抑制脂质过氧化过程实现的。
Objective To study the effect of extract of Cassia seed on the oxidative stress in mice induced by potassium bromate (KBrO3) / Salmonella typhimurium lipopolysaccharide (LPS). Methods Mouse model of oxidative stress was induced by injection of 2 mg.kg-1 LPS into the tail vein of mice and intraperitoneal injection of 200 mg.kg-1 KBrO3. 50, 150 and 450 mg.kg-1 Cassia seed extract were administrated with LPS / KBrO3 at the same time and 3 times before gavage respectively. The control group was orally administered with the same volume of water. Six hours after administration of LPS / KBrO3, the mice lens was taken under ether anesthesia to measure the antioxidant capacity. Results After intraperitoneal injection of 200 mg.kg-1 KBrO3 or tail vein injection of 2 mg.kg-1 LPS for 6 h, the level of glutathione (GSH) was decreased in the lens of mice and significantly decreased when KBrO3 was administered with LPS Induced oxidative stress in mouse lens. The intragastric administration of 50, 150 and 450 mg.kg-1 Cassia seed extracts could effectively decrease the GSH content, increase the antioxidant capacity index (ORAC) and decrease the content of malondialdehyde (MDA) in mouse lens tissue induced by KBrO3 / LPS, Level, and showed a certain amount of effective relationship. Conclusion Cassia seed extract can improve peroxisome state in mouse lens, its mechanism may be through the elimination of free radicals and inhibition of lipid peroxidation process.