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目的探讨饮用水有机提取物致大鼠肝脏DNA及蛋白质的氧化损伤情况。方法采集某地管网末梢水,利用固相萃取法提取水样中有机污染物,对40只SD大鼠进行经口灌胃亚慢性染毒(12周)。设立溶剂对照组(玉米油)和3个染毒组(剂量分别为5 L/kg BW·d、20 L/kg BW·d、80 L/kg BW·d)。8羟基脱氧鸟苷(8-OHd G)采用酶联免疫法(ELISA)、蛋白质羰基(PCO)采用2,4-二硝基苯肼(DNPH)比色法、蛋白定量采用微量酶标法检测。结果随染毒剂量的增加,大鼠血清8-OHd G含量呈上升趋势,与溶剂对照组比较,中、高剂量组的升高差异有统计学意义(P<0.05);大鼠肝组织8-OHd G含量亦在中、高剂量组明显升高(P<0.05)。随染毒剂量的增加,大鼠肝组织PCO含量呈上升趋势,与溶剂对照组比较,中、高剂量组的升高差异有统计学意义(P<0.05)。相关性分析显示,大鼠血清8-OHd G含量、肝组织8-OHd G含量及肝组织PCO含量均与有机提取物染毒剂量呈高度正相关关系(相关系数分别为0.9383,0.8631和0.9136,均P<0.05),同时大鼠血清8-OHd G含量与肝组织8-OHd G含量亦呈高度正相关关系(相关系数为0.9843,P<0.05)。结论饮用水有机提取物较高剂量接触可诱发大鼠肝脏DNA及蛋白质的氧化损伤,此可能为饮用水有机提取物肝脏毒性重要机制之一。
Objective To investigate the oxidative damage of rat liver DNA and protein induced by organic extracts of drinking water. Methods A total of 40 SD rats were sub-chronicly treated with oral gavage (12 weeks) by collecting the peripheral water of a pipe network and extracting organic pollutants by solid-phase extraction. A solvent control group (corn oil) and three exposure groups (5 L / kg BW · d, 20 L / kg BW · d, 80 L / kg BW · d) were set up. 8-OHd G was detected by enzyme-linked immunosorbent assay (ELISA), protein carbonyl (PCO) by 2,4-dinitrophenylhydrazine (DNPH) colorimetric assay, . Results Compared with the solvent control group, the content of 8-OHd G in serum of the rats increased with the increase of the exposure dose. There was a significant difference between the medium and high dose groups (P <0.05) The content of -OHd G also increased significantly in medium and high dose groups (P <0.05). With the increase of exposure dose, the content of PCO in rat liver tissue showed an upward trend. Compared with the solvent control group, the increase of PCO level was statistically significant (P <0.05). Correlation analysis showed that serum 8-OHdG content, liver tissue 8-OHdG content and liver tissue PCO content were highly positively correlated with the dose of organic extract (correlation coefficients were 0.9383,0.8631 and 0.9136 respectively, (All P <0.05). Meanwhile, there was a positive correlation between serum 8-OHd G and the content of 8-OHd G in rat liver (correlation coefficient: 0.9843, P <0.05). Conclusion Higher doses of organic extract of drinking water can induce oxidative damage of DNA and protein in rat liver, which may be one of the important mechanisms of liver toxicity of organic extracts of drinking water.